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油菜种子含油量调控基因首次被克隆
油菜种子含油量调控基因首次被克隆
2019 - 02 - 13
日前，《分子植物》（Molecular Plant）在线发表了中国农业科学院油料作物研究所王汉中院士团队最新成果。该团队在国际上首次成功克隆了农作物种子性状的第一个细胞质调控基因 orf188，并揭示了该基因调控油菜种子高含油量的作用机制。该成果为油菜高含油量育种提供了新途径，为育种过程中杂交母本的选择提供了理论支撑，对于农作物不同类型细胞质的应用具有重要的指导意义。含油量是油菜产油量最重要的构成因子之一，随着现代育种对含油量潜力的不断挖掘，提高品种的含油量越来越困难。该团队在油菜含油量遗传研究的基础上，从细胞质效应着手对含油量母体调控新机制研究取得突破，筛选到两个对含油量具有极显著细胞质正负效应的品种，鉴定出特异基因 orf188 控制细胞质的含油量正效应，过表达该基因可以大幅度提升含油量。研究表明，orf188 为 nap（nap-like）型油菜品种所特有基因，是一个新近进化形成的嵌合基因。该团队十余年来围绕油菜种子性状的母体调控进行系统研究，此次对细胞质基因调控种子含油量机理的揭示，是该团队继发现角果皮发育调控种子粒重和含油量之后发现的又一条母体调控新途径，进一步丰富和完善了种子性状的母体调控理论，也为其他农作物性状细胞质效应的解析提供了借鉴。相关论文信息：DOI:10.1016/j.molp.2019.01.012来源：中国科学报转载：生物360
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AI技术在医学影像中的应用现状及行业发展情况分析
AI技术在医学影像中的应用现状及行业发展情况分析
2019 - 06 - 14
随着技术飞速发展、医学数据的持续扩增以及硬件设备的不断提升，人工智能和医疗的结合方式越来越多样化。目前AI在医疗领域中的落地的应用场景主要有医学影像、智能诊疗、智能导诊、智能语音、健康管理、病例分析、医院管理、新药研发和医疗机器人等，其中在医学影像中的应用最为广泛。一、影像医学发展现状医学影像是医生完成诊断的主要依据，通过对影像的分析和比较，从而完成有依据的诊断。但是在实际过程中，往往会存在以下问题：（1）影像学诊断人才资源紧缺。医疗机构普遍缺乏高水平的影像医师，在疾病诊断时往往会发生同病异影，异病同影等情况。（2）传统定性分析存在诊断误差。医生普遍擅长定性分析，很多微小的定量变化无法通过肉眼判断，很难做到定量分析。（3）医生阅片时间长。目前的影像呈现方式为数据和图像，而不是最有效的信息，很大程度上限制了医生的人工阅片速度。二、AI+医学影像助力疾病诊断通过引入人工智能可有效解决部分问题，目前人工智能在医学影像领域的应用方向主要以下几类：1. 影像设备的图像重建AI可以通过算法的图像映射技术，将采集的少量信号恢复出与全采样图像同样质量的图像，而且使用图像重建技术，可以由低剂量的CT和PET图像重建得到高剂量质量图像。这样在满足临床诊断需求的同时，还能够降低辐射的风险。2. 智能辅助诊断疾病（1）智能辅助诊断肺部疾病国内应用AI+CT影像最为成熟的领域在肺结节的识别上。AI能够有效识...
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研究揭示多动症儿童前瞻记忆功能的特点
研究揭示多动症儿童前瞻记忆功能的特点
2019 - 12 - 25
前瞻记忆是指个体记住未来要完成任务的能力。儿童进入学校后，前瞻记忆任务逐渐增多，对前瞻记忆能力的要求也逐渐增高。前瞻记忆任务成功的关键在于当个体看到线索时能主动提取并执行意图。前瞻记忆线索分为三种，时间线索（如周六晚上7点记得参加朋友的生日聚会）、事件线索（如路过超市记得买水果）和活动结束线索（如写完作业记得找家长签字）。三种线索的提取难度存在差异，这可能与其对注意资源的需求不同有关。较之正常发育的儿童，多动症儿童在日常活动中常有注意力不集中和健忘的表现，提示其前瞻记忆能力可能存在缺损。然而，对多动症儿童前瞻记忆功能的研究较少，更缺乏研究直接考察多动症儿童在三种线索条件下的前瞻记忆表现及其认知加工特点。因此，中国科学院心理研究所心理健康重点实验室神经心理和应用认知神经科学（NACN）实验室陈楚侨研究组与北医六院医生钱英、潍坊医学院教授王艳郁合作考察了多动症儿童在时间、事件和活动结束线索条件下的前瞻记忆表现。该研究招募了28名临床诊断的多动症儿童和28名年龄和性别匹配的正常发育儿童，采用钓鱼游戏任务(Yang， Chan， Shum， 2011)测查儿童在三种线索条件下的前瞻记忆功能。该任务采取经典的双任务范式，主任务要求儿童钓鱼，并同时关注前瞻记忆线索，在线索出现时儿童需要停止钓鱼并执行前瞻记忆任务。在时间线索条件下，儿童要记得每到整分钟就喂猫吃鱼；在事件线索条件下，儿童看到特殊条...
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LIVE/DEAD™ Fixable Green Dead Cell Stain Kit, for 488 nm excitation
产品名称： LIVE/DEAD™ Fixable Green Dead Cell Stain Kit, for 488 nm excitation
型号：
时间： 2019 - 04 - 22
描述The LIVE/DEAD™ Fixable Green Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. This kit has been optimized and validated for use with a blue laser flow cytometer.• Stable—dyes are freeze dried in separate vials to maintain stability• Robust—staining pattern is the same before and after fixation• Bright signal—allows...
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Dead Cell Apoptosis Kit with Annexin V FITC and PI, for flow cytometry
产品名称： Dead Cell Apoptosis Kit with Annexin V FITC and PI, for flow cytometry
型号：
时间： 2019 - 04 - 18
描述This product detects the externalization of phosphatidylserine in apoptotic cells using recombinant annexin V conjugated to green-fluorescent FITC dye and dead cells using propidium iodide (PI). Propidium iodide stains necrotic cells with red fluorescence. After treatment with both probes, apoptotic cells show green fluorescence, dead cells show red and green fluorescence, and live cells show little or no fluorescence.
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SYTOX™ Green Nucleic Acid Stain - 5 mM Solution in DMSO
产品名称： SYTOX™ Green Nucleic Acid Stain - 5 mM Solution in DMSO
型号：
时间： 2019 - 04 - 18
描述SYTOX® Green nucleic acid stain is an excellent green-fluorescent nuclear and chromosome counterstain that is impermeant to live cells, making it a useful indicator of dead cells within a population.•  Impermeant to live cells•  Excitation/Emission: 504/523 nm•  Use with 488 Argon-ion laser•  Works with mammalian cells and both Gram-positive and Gram-negative bacteriaSimple Viability Determination SYTOX® Green allows quick determination of cell viability when usin...
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Premo™ FUCCI Cell Cycle Sensor (BacMam 2.0)
产品名称： Premo™ FUCCI Cell Cycle Sensor (BacMam 2.0)
型号：
时间： 2019 - 04 - 18
描述Premo™ FUCCI Cell Cycle Sensor is a fluorescent, two-color sensor of cell cycle progression and division in live cells. It allows accurate and sensitive cell cycle analysis of individual cells or a population of cells by fluorescence microscopy, flow cytometry, or high-content imaging.At 2 x 200 µL, this is a smaller pack size version (1/5 volume) of the popular full-size product and perfect for testing its performance in your system.The Premo™ FUCCI Cell Cycle Sensor is delivered by high...
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CountBright™ Absolute Counting Beads, for flow cytometry
产品名称： CountBright™ Absolute Counting Beads, for flow cytometry
型号：
时间： 2019 - 04 - 18
描述CountBright™ absolute counting beads are a calibrated suspension of microspheres that are brightly fluorescent across a wide range of excitation and emission wavelengths (UV to 635 nm excitation and 385-800 nm emission). CountBright™ absolute counting beads are mixed with the cell sample and assayed via flow cytometry. By comparing the ratio of bead events to cell events, absolute numbers of cells in the sample can be calculated. Because CountBright™ beads are mixed in the test sample, absolut...
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CellTrace™ CFSE Cell Proliferation Kit, for flow cytometry
产品名称： CellTrace™ CFSE Cell Proliferation Kit, for flow cytometry
型号：
时间： 2019 - 04 - 17
描述CellTrace™ CFSE Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry.• Superior performance—bright, single-peak staining enables visualization of multiple generations• Long-term signal stability—well-retained in cells for several days post stain• Non-toxic—staining does not adversely effect cell health• Simple, robust staining protocolView a selection guide for all CellTrace™ Cell Proliferation Kits fo...
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CellTrace™ CFSE Cell Proliferation Kit - For Flow Cytometry
产品名称： CellTrace™ CFSE Cell Proliferation Kit - For Flow Cytometry
型号：
时间： 2019 - 04 - 17
描述CellTrace™ CFSE 细胞增殖试剂盒 (CellTrace™ CFSE Cell Proliferation Kit) 以方便的一次性瓶装形式提供用途广泛，稳定性高的细胞示踪剂。 羧基荧光素二醋酸盐琥珀酰亚胺酯，常称为 CFSE，可被动扩散进入细胞。 这种试剂没有颜色和荧光，直至细胞内的酯酶去除其醋酸盐基团，产生能发出明亮荧光的羧基荧光素琥珀酰亚胺酯。 琥珀酰亚胺酯与细胞内氨基反应，形成稳定且能用醛固定剂固定的荧光偶联物。 此试剂盒包含十份一次性瓶装的 CFSE，因此进行小规模实验时无需制备过量的易变质 CFSE 储存液。 此试剂盒还包括高质量的 DMSO（二甲基亚砜）和详细的实验方案。For Research Use Only. Not for use in diagnostic procedures.图表Suppressive capacity of isolated Treg cells.Suppressive capacity of isolated Treg cells. (A) CD4+ CD25–cells were stained with Ca...
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Click-iT™ EdU Pacific Blue™ Flow Cytometry Assay Kit
产品名称： Click-iT™ EdU Pacific Blue™ Flow Cytometry Assay Kit
型号：
时间： 2019 - 04 - 17
描述The Click-iT® EdU Pacific Blue™ Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells as compared to traditional BrdU methods. Newly synthesized DNA is analyzed using the 405 nm laser of the flow cytometer.• Accurate—superior results compared to BrdU assays• Fast—results in as little as 90 minutes• Economical—more assays per kitView selection guide for all Click-iT™ EdU and Click-iT™ Plus EdU assays for flow c...
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Attune™ NxT Acoustic Focusing Cytometer, blue
产品名称： Attune™ NxT Acoustic Focusing Cytometer, blue
型号：
时间： 2019 - 04 - 17
描述The 2018 Attune NxT Acoustic Focusing Cytometer has expanded functionalities to drive your research forward. Designed and developed to remove common barriers associated with flow cytometry, the evolutionary capabilities of the Attune NxT flow cytometer offer adaptable optical configuration options so you can get the most out of your multicolor analysis.• Free Attune NxT software with researcher-inspired upgrades• New green laser option for expanded utility and fluorochrome options• Simple push...
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Attune™ NxT Acoustic Focusing Cytometer, blue/red
产品名称： Attune™ NxT Acoustic Focusing Cytometer, blue/red
型号：
时间： 2019 - 04 - 17
描述The 2018 Attune NxT Acoustic Focusing Cytometer has expanded functionalities to drive your research forward. Designed and developed to remove common barriers associated with flow cytometry, the evolutionary capabilities of the Attune NxT flow cytometer offer adaptable optical configuration options so you can get the most out of your multicolor analysis.• Free Attune NxT software with researcher-inspired upgrades• New green laser option for expanded utility and fluorochrome options• Simple push...
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Attune™ NxT Acoustic Focusing Cytometer, blue/red/violet
产品名称： Attune™ NxT Acoustic Focusing Cytometer, blue/red/violet
型号：
时间： 2019 - 04 - 17
描述The 2018 Attune NxT Acoustic Focusing Cytometer has expanded functionalities to drive your research forward. Designed and developed to remove common barriers associated with flow cytometry, the evolutionary capabilities of the Attune NxT flow cytometer offer adaptable optical configuration options so you can get the most out of your multicolor analysis.• Free Attune NxT software with researcher-inspired upgrades• New green laser option for expanded utility and fluorochrome options• Simple push...
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Attune™ NxT Acoustic Focusing Cytometer, blue/red/violet/yellow
产品名称： Attune™ NxT Acoustic Focusing Cytometer, blue/red/violet/yellow
型号：
时间： 2019 - 04 - 17
描述The 2018 Attune NxT Acoustic Focusing Cytometer has expanded functionalities to drive your research forward. Designed and developed to remove common barriers associated with flow cytometry, the evolutionary capabilities of the Attune NxT flow cytometer offer adaptable optical configuration options so you can get the most out of your multicolor analysis.• Free Attune NxT software with researcher-inspired upgrades• New green laser option for expanded utility and fluorochrome options• Simple push...
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