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冠状病毒简史：带你了解冠状病毒的前世今生！
冠状病毒简史：带你了解冠状病毒的前世今生！
2020 - 04 - 30
2020年4月28日讯 /生物谷BIOON /——我们大多数人一生中至少会感染一次冠状病毒。对于许多人来说，这可能是一个令人担忧的事实，尤其是那些只听说过一种冠状病毒SARS-CoV-2的人。冠状病毒远远不止SARS-CoV-2这一种。冠状病毒实际上是数百种病毒的一个家族。其中大多数感染动物，如蝙蝠、鸡、骆驼和猫。有时，感染一个物种的病毒会发生变异，从而开始感染另一个物种。这被称为'跨物种传播'或'溢出效应'。第一种冠状病毒是在20世纪30年代的鸡身上发现的。直到20世纪60年代首次发现人类冠状病毒，人类才花了几十年的时间。迄今为止，有7种冠状病毒有能力导致人类患病。四种是地方性的(通常在特定人群或某个地区发现)，通常引起轻微的疾病，但三种可能引起严重得多甚至致命的疾病。图片来源：https://cn.bing.com普通感冒冠状病毒在世界各地都有，大约有10-15%的普通感冒是由冠状病毒引起的，大部分发生在冬季。导致人类轻度至中度疾病的冠状病毒称为：229E、OC43、NL63和HKU1。首次发现能够感染人类的冠状病毒是229E和OC43。这两种病毒通常会导致普通的感冒，很少会导致严重的疾病。它们通常与其他呼吸道感染同时被发现。当在病人体内发现多种病毒或病毒和细菌时，这就称为合并感染，并可导致更严重的疾病。2004年，荷兰首次在患有细支气管炎(一种...
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一次编辑数百种基因！遗传学大牛George Church发布基因编辑新方法
一次编辑数百种基因！遗传学大牛George Church发布基因编辑新方法
2018 - 05 - 25
基因编辑技术帮助人们实现了“编辑生命”的愿望，但该技术自身存在的局限性也限制了其广泛应用，此前人们的研究热情也多集中在编辑的效率、准确性与安全性，但常规的基因编辑技术在一个反应中仅能对一个或数个基因进行操作，且通常会在基因组中留下额外的不需要的序列修改，在大型研究中，已有的基因编辑技术远远无法满足大量不同基因的编辑需求。为解决上述问题，哈佛大学George Church教授领导的研究团队开发了一种基于CRISPR-Cas9的新型高通量基因编辑方法：guide+donor，该方法可以在单个酿酒酵母中同时精确改变数百个不同基因，编辑效率达到80％至100％，还能在生物体内选择特定行为细胞，进行定向基因编辑和修复。guide+donor除帮助构建大型文库外，还能进行基因功能分析，发现一些未知的基因突变和功能。5月21日，相关研究成果发表在著名学术期刊Nature Biotechnology上，文章题为“High-throughput creation and functional profiling of DNA sequence variant libraries using CRISPR–Cas9 in yeast”。George Church教授人类的基因变异通常是点突变导致，为在不干扰其他潜在变异的情况下重构酿酒酵母中的某些变异，CRISPR-Cas9技术借助sgRNA精确靶向D...
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揭秘细菌挑食之谜
揭秘细菌挑食之谜
2019 - 03 - 22
给你一个冰淇淋和一块蛋糕，你先吃哪个？一块面包和一个牛油果呢？两个一块吃吗？1941 年微生物学家 Jacques Monod 给细菌提出了同样的问题。他给大肠杆菌同时提供两种食物（碳源），结果看到了两类不同的现象：对某一些食物组合（比麦芽糖和苹果酸），细菌两种同时吃，而对另一些组合（比如葡萄糖和乳糖），细菌要先吃完一种（葡萄糖），然后再会去吃另一种（乳糖）。随后 Monod 和 François Jacob 一道，找出了细菌在有葡萄糖和乳糖的情况下只吃葡萄糖的分子机制—乳糖操纵子：在这两种糖都存在的时候，细胞不会去表达运输和消化乳糖的相关基因，并获得了 1965 年的诺贝尔生理学奖。可是为什么细菌要挑食呢？为什么对于有些食物的组合它们又不挑呢？北京大学汤超教授团队发现细菌的这一套进食策略能够使它们最优化的生长。如果哪个菌种想任性一把，同时吃葡萄糖和乳糖，或者对麦芽糖和苹果酸挑食，那么它们的代价就是生长速率的降低，进而面临被淘汰的危险。结合代谢网络的拓扑特征和细胞内蛋白资源的优化分配，研究团队的理论模型定量的解释了细菌面对多种碳源的进食策略。在两类碳源同时被细菌消耗的情况下，模型预测并用实验验证了细菌内不同氨基酸上的碳原子来自两种碳源的比例。这个例子进一步说明了在看似复杂的生命现象中，存在简洁的原理与定量的规律。相关文章发表在《自然 - 通讯》杂志上。北京大学定量生物学中...
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EVOS™ 50X Oil Objective, achromat, coverslip-corrected
产品名称： EVOS™ 50X Oil Objective, achromat, coverslip-corrected
型号：
时间： 2019 - 04 - 15
描述This achromat objective is ideal for general applications. This is a coverslip-corrected objective that is optimized for imaging through #1.5 coverslips. All EVOS® objectives offer outstanding optical performance from visible light to near infrared light. The extensive choice of objectives satisfies needs across the spectrum of magnifications and optical specifications.Additional characteristics of this EVOS® objective:• Magnification: 50X• Numerical Aperture: 0.95• Working Distance:...
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EVOS™ M7000 Imaging System
产品名称： EVOS™ M7000 Imaging System
型号：
时间： 2019 - 04 - 15
描述The EVOS M7000 Imaging System is a high-performance, fully automated, inverted, multi-channel fluorescence and transmitted light imaging system. It was developed to meet today’s expectations for image quality, user interface interactions, data generation speed and flexibility, and end-user value. The powerful and time-saving acquisition tools with enhanced autofocus algorithms and automated routines for microwell plate assays, time-lapse live cell imaging, area scanning in time lapse and Z-sta...
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EVOS™ FL Imaging System
产品名称： EVOS™ FL Imaging System
型号：
时间： 2019 - 04 - 15
描述The EVOS® FL Imaging System is a fully integrated, digital, inverted imaging system for four-color fluorescence and transmitted-light applications. It is powerful, yet easy-to-use and delivers high-definition images with exceptional convenience. The unique light cubes, sensitive camera, and precision-engineered optical system make the EVOS® FL system ideal for both demanding and routine fluorescence imaging applications.The EVOS® FL Imaging System offers you these important ad...
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EVOS™ Onstage Master Plate
产品名称： EVOS™ Onstage Master Plate
型号：
时间： 2019 - 04 - 15
描述The EVOS® Onstage Master Plate is part of the EVOS® Onstage Incubator and holds the EVOS® Onstage Vessel Holder. The EVOS® Onstage Incubator is an environmental chamber designed specifically for use with the EVOS® FL Auto Imaging System. This master plate plus vessel holder design, with vessel holders available that accommodate various types of cell culture vessels, provides flexibility in your approach to extended time-lapse imaging experiments under precisely co...
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EVOS™ Onstage Vessel Holder, two 25-mL flasks
产品名称： EVOS™ Onstage Vessel Holder, two 25-mL flasks
型号：
时间： 2019 - 04 - 15
描述EVOS® Onstage vessel holders maximize your choice of cell culture vessels when conducting time-lapse imaging experiments using the EVOS® FL Auto Imaging System equipped with an EVOS® Onstage Incubator. EVOS® Onstage vessel holders allow a perfect fit of your micro-well plates or cell culture flasks/dishes to the master plate of the EVOS® Onstage Incubator for increased precision in sample alignment. This particular vessel holder accommodates two 25-mL T-flasks. With ...
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EVOS™ Onstage Vessel Holder, multi-well plates
产品名称： EVOS™ Onstage Vessel Holder, multi-well plates
型号：
时间： 2019 - 04 - 15
描述EVOS® Onstage vessel holders maximize your choice of cell culture vessels when conducting time-lapse imaging experiments using the EVOS® FL Auto Imaging System equipped with an EVOS® Onstage Incubator. EVOS® Onstage vessel holders allow a perfect fit of your micro-well plates or cell culture flasks/dishes to the master plate of the EVOS® Onstage Incubator for increased precision in sample alignment. This particular vessel holder accommodates multi-well plates (dimensi...
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EVOS™ Vessel Holder for Automated Stage, two 25 x 75 mm slides
产品名称： EVOS™ Vessel Holder for Automated Stage, two 25 x 75 mm slides
型号：
时间： 2019 - 04 - 15
描述EVOS® vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS® imaging systems for increased precision in sample alignment. This particular vessel holder securely holds two 25 x 75 mm standard or chamber microscope slides and is specific for the automated stage of the EVOS® FL Auto Imaging System. It is just one of many easy-to-change vessel holders and stage plates available for this instrument plat...
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EVOS™Vessel Holder, Greiner/BD T-25 flask
产品名称： EVOS™Vessel Holder, Greiner/BD T-25 flask
型号：
时间： 2019 - 04 - 15
描述EVOS® vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS® imaging systems for increased precision in sample alignment. This particular vessel holder accommodates one Greiner/BD T-25 flask. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of ...
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EVOS™ Vessel Holder, universal
产品名称： EVOS™ Vessel Holder, universal
型号：
时间： 2019 - 04 - 15
描述EVOS® vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS® imaging systems for increased precision in sample alignment. This particular vessel holder has a 25.4 mm opening and accommodates most vessel types. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samp...
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EVOS™ Vessel Holder, Greiner T-75 flask
产品名称： EVOS™ Vessel Holder, Greiner T-75 flask
型号：
时间： 2019 - 04 - 15
描述EVOS® vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS® imaging systems for increased precision in sample alignment. This particular vessel holder accommodates one Greiner T-75 flask. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of ves...
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EVOS™ Vessel Holder, Hemocytometer
产品名称： EVOS™ Vessel Holder, Hemocytometer
型号：
时间： 2019 - 04 - 15
描述EVOS® vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS® imaging systems for increased precision in sample alignment. This particular vessel holder accommodates a hemocytometer. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of vessel hol...
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EVOS™ Vessel Holder, Nunc™ T-75 flask
产品名称： EVOS™ Vessel Holder, Nunc™ T-75 flask
型号：
时间： 2019 - 04 - 15
描述EVOS® vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS® imaging systems for increased precision in sample alignment. This particular vessel holder accommodates one Nunc® T-75 flask. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of ...
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