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2020 - 02 - 21
2020年2月16日讯 /生物谷BIOON /--国家指南建议前列腺癌患者多吃蔬菜,这可能会减少癌症的进展和死亡。但在一项III期随机临床试验中,前列腺癌患者被指定每天吃7份或更多的蔬菜和水果,却没有发现额外的微量营养元素摄入的保护作用。'这些数据表明,尽管科学和公众舆论普遍认为需要吃蔬菜,但多吃蔬菜缺确实不会改变前列腺癌的病程。据我们所知,它不会抑制或治愈它。然而,虽然多吃水果和蔬菜的健康饮食和多做运动不一定能治愈癌症,但它可能使身体更强壮、更健康,这可能有助于患者忍受癌症治疗。'图片来源:Wikipedia这项研究近日发表在《Journal of the American Medical Association》上,,由加州大学圣地亚哥分校Moores癌症中心和Roswell Park综合癌症中心的研究人员完成,招收了478名年龄在50到80岁男性。这些患者被诊断为早期前列腺腺癌,并参与了一个积极的监测项目,在该项目中,患者推迟立即治疗,直到病情恶化。患者被随机分为两组,一组接受关于饮食和前列腺癌的书面信息,另一组接受电话咨询的行为干预项目,该项目鼓励参与者食用类胡萝卜素含量高的食物,如绿叶蔬菜、胡萝卜和西红柿,以及十字花科蔬菜,如西兰花和卷心菜。两组都接受了两年的监测。被分配到干预组的患者增加了水果和蔬菜的摄入量,达到了有统计学意义的程度,明显多于对照组患者。...
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2018 - 05 - 25
基因编辑技术帮助人们实现了“编辑生命”的愿望,但该技术自身存在的局限性也限制了其广泛应用,此前人们的研究热情也多集中在编辑的效率、准确性与安全性,但常规的基因编辑技术在一个反应中仅能对一个或数个基因进行操作,且通常会在基因组中留下额外的不需要的序列修改,在大型研究中,已有的基因编辑技术远远无法满足大量不同基因的编辑需求。为解决上述问题,哈佛大学George Church教授领导的研究团队开发了一种基于CRISPR-Cas9的新型高通量基因编辑方法:guide+donor,该方法可以在单个酿酒酵母中同时精确改变数百个不同基因,编辑效率达到80%至100%,还能在生物体内选择特定行为细胞,进行定向基因编辑和修复。guide+donor除帮助构建大型文库外,还能进行基因功能分析,发现一些未知的基因突变和功能。5月21日,相关研究成果发表在著名学术期刊Nature Biotechnology上,文章题为“High-throughput creation and functional profiling of DNA sequence variant libraries using CRISPR–Cas9 in yeast”。George Church教授人类的基因变异通常是点突变导致,为在不干扰其他潜在变异的情况下重构酿酒酵母中的某些变异,CRISPR-Cas9技术借助sgRNA精确靶向D...
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2019 - 03 - 15
基因组的遗传信息得以表达,首先需要 RNA polymerase (RNAP) 以 DNA 为模板合成 RNA。基因转录不仅是基因表达第一步,还是基因表达的主要调控步骤。对 RNAP 分子机器结构、运行机理以及调控机制的研究能够回答基因表达调控的基础生物学问题。在转录起始阶段,细菌的 RNAP 与转录起始σ因子形成复合物,依次执行启动子双链 DNA 的识别、解链以及 RNA 起始合成等关键步骤。细菌 RNAP 通过与多个σ因子结合特异性调控基因转录,其中 Extra-Cytoplasmic Function(ECF)σ因子是细菌中种类最多的一类σ因子,它可以感受细菌胞内外环境变化,起始特异性的基因转录。ECF σ因子赋予细菌适应逆境的能力,对于致病菌的致病性和耐药性尤为重要。以结核分枝杆菌(Mycobacterium tuberculosis)为例,其 RNAP 分别与 10 种 ECF σ因子结合,通过识别特异启动子序列启动相应基因表达,多个 ECF σ因子与结核分枝杆菌的致病、侵染以及耐药直接相关。3 月 11 日,国际学术期刊《自然 - 通讯》(Nature communications)在线发表了中国科学院分子植物科学卓越创新中心 / 植物生理生态研究所 / 中科院合成生物学重点实验室张余研究组题为 Structure basis for transcription ini...
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时间: 2019 - 04 - 17
描述The 2018 Attune NxT Acoustic Focusing Cytometer has expanded functionalities to drive your research forward. Designed and developed to remove common barriers associated with flow cytometry, the evolutionary capabilities of the Attune NxT flow cytometer offer adaptable optical configuration options so you can get the most out of your multicolor analysis.• Free Attune NxT software with researcher-inspired upgrades• New green laser option for expanded utility and fluorochrome options• Simple push...
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时间: 2019 - 04 - 17
描述7-aminoactinomycin D (7-AAD) is a fluorescent intercalator that undergoes a spectral shift upon association with DNA. 7-AAD/DNA complexes can be excited by the 488 nm laser and has an emission maxima of 647 nm, making this nucleic acid stain useful for multicolor fluorescence microscopy and flow cytometry. 7-AAD appears to be generally excluded from live cells, but can be used with cells that have been fixed and permeabilized. 7-AAD has been used for cell cycle analysis by flow cytometry....
产品名称: Dynabeads™ CD45
型号:
时间: 2019 - 04 - 17
DescriptionDynabeads® CD45 are superparamagnetic beads covalently coupled to anti-human CD45 antibody that enable isolation or depletion of CD45+ leucocytes directly from whole blood, buffy coat, or MNC suspensions. Dynabeads® CD45 can also be used to enrich epithelial tumor cells. The CD45 antigen is weakly expressed on myeloid cells. To efficiently deplete or isolate all leucocytes including myeloid cells, use Dynabeads® CD15 (Cat. No. 11137D) in combination with Dynabeads®...
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时间: 2019 - 04 - 15
描述This achromat objective is ideal for general applications. This is a coverslip-corrected objective that is optimized for imaging through #1.5 coverslips. All EVOS® objectives offer outstanding optical performance from visible light to near infrared light. The extensive choice of objectives satisfies needs across the spectrum of magnifications and optical specifications.Additional characteristics of this EVOS® objective:• Magnification: 50X• Numerical Aperture: 0.95• Working Distance:...
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时间: 2019 - 04 - 15
描述The EVOS M7000 Imaging System is a high-performance, fully automated, inverted, multi-channel fluorescence and transmitted light imaging system. It was developed to meet today’s expectations for image quality, user interface interactions, data generation speed and flexibility, and end-user value. The powerful and time-saving acquisition tools with enhanced autofocus algorithms and automated routines for microwell plate assays, time-lapse live cell imaging, area scanning in time lapse and Z-sta...
型号:
时间: 2019 - 04 - 15
描述The EVOS® FL Imaging System is a fully integrated, digital, inverted imaging system for four-color fluorescence and transmitted-light applications. It is powerful, yet easy-to-use and delivers high-definition images with exceptional convenience. The unique light cubes, sensitive camera, and precision-engineered optical system make the EVOS® FL system ideal for both demanding and routine fluorescence imaging applications.The EVOS® FL Imaging System offers you these important ad...
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时间: 2019 - 04 - 15
描述The EVOS® Onstage Master Plate is part of the EVOS® Onstage Incubator and holds the EVOS® Onstage Vessel Holder. The EVOS® Onstage Incubator is an environmental chamber designed specifically for use with the EVOS® FL Auto Imaging System. This master plate plus vessel holder design, with vessel holders available that accommodate various types of cell culture vessels, provides flexibility in your approach to extended time-lapse imaging experiments under precisely co...
型号:
时间: 2019 - 04 - 15
描述EVOS® Onstage vessel holders maximize your choice of cell culture vessels when conducting time-lapse imaging experiments using the EVOS® FL Auto Imaging System equipped with an EVOS® Onstage Incubator. EVOS® Onstage vessel holders allow a perfect fit of your micro-well plates or cell culture flasks/dishes to the master plate of the EVOS® Onstage Incubator for increased precision in sample alignment. This particular vessel holder accommodates two 25-mL T-flasks. With ...
型号:
时间: 2019 - 04 - 15
描述EVOS® Onstage vessel holders maximize your choice of cell culture vessels when conducting time-lapse imaging experiments using the EVOS® FL Auto Imaging System equipped with an EVOS® Onstage Incubator. EVOS® Onstage vessel holders allow a perfect fit of your micro-well plates or cell culture flasks/dishes to the master plate of the EVOS® Onstage Incubator for increased precision in sample alignment. This particular vessel holder accommodates multi-well plates (dimensi...
型号:
时间: 2019 - 04 - 15
描述EVOS® vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS® imaging systems for increased precision in sample alignment. This particular vessel holder securely holds two 25 x 75 mm standard or chamber microscope slides and is specific for the automated stage of the EVOS® FL Auto Imaging System. It is just one of many easy-to-change vessel holders and stage plates available for this instrument plat...
型号:
时间: 2019 - 04 - 15
描述EVOS® vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS® imaging systems for increased precision in sample alignment. This particular vessel holder accommodates one Greiner/BD T-25 flask. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of ...
型号:
时间: 2019 - 04 - 15
描述EVOS® vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS® imaging systems for increased precision in sample alignment. This particular vessel holder has a 25.4 mm opening and accommodates most vessel types. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samp...
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