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1
2018 - 07 - 20
一种非常复杂的治疗一些乳腺癌和卵巢癌的方法就是使用一类叫做PARP抑制剂的药物。PARP抑制剂旨在利用让发生某些突变的肿瘤特别致命的缺陷。然而,这种靶向癌症治疗方法有时会失败,科学家们迫切地想要知道其中的原因。 如今,在一项新的研究中,美国洛克菲勒大学的Titia de Lange教授及其团队对这种耐药性机制提供了新的认识,并且为抵抗这种耐药性提供了新的希望。他们发现了由基因BRCA1发生的错误引发的一些癌症逃避旨在杀死它们的定制药物的分子机制。这一发现也挑战了之前针对这些PARP抑制剂成功地或未能给患者带来益处的机制作出的猜测。相关研究结果于2018年7月18日在线发表在Nature期刊上,论文标题为“53BP1–RIF1–shieldin counteracts DSB resection through CST- and Polα-dependent fill-in”。图片来自Laboratory of Cell Biology and Genetics at The Rockefeller University。他们的发现有助于解释为什么某些癌症会对PARP抑制剂作出反应,而其他癌症却没有---这种认识最终可能有助于改善对患者的治疗。 缺陷和机会 专家们预测今年将有大约288000例乳腺癌和卵巢癌新确诊病例。这些癌症的很大一部分是由人类基因组中的两个最臭名昭着的基因--- ...
2
2019 - 05 - 23
2019年5月22日 讯 /生物谷BIOON/ --早稻田大学Toshio Ohshima教授的一项新研究发现,抑制塌陷反应介质蛋白2(CRMP2)(一种微管结合蛋白)的磷酸化可以抑制神经纤维的退化,促进视神经损伤后的再生。最近在《Scientific Reports》杂志上发表的这项研究结果可以为视神经病变患者开发新型治疗方法。青光眼患者视野中会出现盲点,并且当视神经恶化时可能导致失明。神经纤维的这种恶化和功能丧失也发生在创伤性神经损伤和神经系统疾病,例如阿尔茨海默病和ALS中。目前,由于轴突再生受到抑制因子的限制,因此不存在在损伤或变性后完全修复视网膜,脊髓和中枢神经系统的其他部分的方法。(图片来源:Www.pixabay.com)在过去的研究中,已经发现了抑制轴突再生的潜在机制,并且认为解决这些机制可以使科学家们在开发新的中枢神经系统损伤治疗方法方面更进一步。CRMP2蛋白质分子起到稳定微管的作用,微管在神经元细胞水平为中枢神经系统提供结构支持,并通过与微管蛋白二聚体结合促进聚合。然而,这些功能通过磷酸化(一种调节神经元蛋白的机制)被各种激酶阻止。“在我们之前的研究中,我们所做的是开发CRMP2敲入小鼠并从基因上抑制其CRMP2磷酸化,”Ohshima教授解释说。 “结果,CRMP2敲入小鼠在脊髓损伤后表现出轴突再生的促进。由此,我们假设在视神经损伤后也可以观察到相同的现象。...
3
2019 - 09 - 29
2019年8月24日讯 /生物谷BIOON /——一项新的研究表明,一种特殊的碳水化合物在调节人体血压方面发挥着重要作用。哥本哈根大学和Rigshospitalet的研究人员用老鼠做了一项新的研究证明了这一点。研究人员认为,这一发现可能对改进高血压药物具有巨大的潜力。高血压和低血压都可能对健康产生不良后果,分别导致心血管疾病和晕厥。现在,研究人员对有助于调节血压的因素有了更多的了解。这项新研究结果发表在《Journal of Biological Chemistry》杂志上。在哥本哈根大学和Rigshospitalet的跨学科合作中,博士生Lasse Holst Hansen在人类身上一种特殊的肽激素上发现了一种特定形式的碳水化合物或糖。此外,在对大鼠的实验中,研究小组发现含有这种糖的肽激素会影响血压的调节。他们希望,他们的研究结果在未来可以用于开发更好的高血压药物。图片来源:https://cn.bing.com'对于开发一种没有副作用(比如晕厥)的现代治疗高血压的方法,这可能是一个非常好的机会。人们早就知道这种肽激素对血压非常重要,但到目前为止还不能用于治疗。这一发现可能只是因为我们跨学科合作、基础和临床研究相结合的结果。'Rigshospitalet临床生物化学系Jens Peter G?tze教授说道。大约五分之一的丹麦人患有高血压。这会增加心血管疾病的风险...
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产品名称:

Premo™ FUCCI Cell Cycle Sensor (BacMam 2.0)

上市日期: 2019-04-18
产品类别: INVITROGEN

规格

Color:Green, Red-Orange
Delivery Method:Baculovirus (BacMam System)
Detection Method:Fluorescent
Emission Class:Visible
Excitation Class:Visible
Excitation⁄Emission (nm):488⁄510 (Green), 555⁄584 (Red)
For Use With (Equipment):Flow Cytometer, Fluorescence Microscope, Fluorescent Imager, High Content Instrument, Tali® Image-Based Cytometer
Form:Liquid
Label or Dye:GFP (EmGFP), RFP (TagRFP)
Product Line:Premo™
Product Size:2 x 200 µL
Quantity:2 vials (200 µl each)
Sub-Cellular Localization:Nucleus
Technique:Fluorescence Intensity
Shipping Condition:Wet Ice


  • 产品描述
  • 产品功能
  • 产品参数

描述

Premo™ FUCCI Cell Cycle Sensor is a fluorescent, two-color sensor of cell cycle progression and division in live cells. It allows accurate and sensitive cell cycle analysis of individual cells or a population of cells by fluorescence microscopy, flow cytometry, or high-content imaging.

At 2 x 200 µL, this is a smaller pack size version (1/5 volume) of the popular full-size product and perfect for testing its performance in your system.

The Premo™ FUCCI Cell Cycle Sensor is delivered by highly efficient BacMam 2.0 technology, enabling cell cycle studies in essentially any cell type. Provided in a ready-to-use format—simply add, incubate, and image. Premo™ FUCCI affords highly efficient transient expression in cell lines, primary cells, and stem cells.

Premo™ FUCCI Cell Cycle Sensor is:

Accurate: Cell-cycle controlled expression of bright GFP and RFP indicators for live cell analysis of individual cells or populations
Highly Efficient: >90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Fast and Convenient: Simply add Premo™ FUCCI Cell Cycle Sensor to your cells in complete medium, incubate overnight, and analyze
Safe: BacMam reagents are non-integrating and non-replicating in mammalian cells, lack observable cytopathic effects, and are suitable for biosafety level (BSL) 1 handling

Study Cell Cycle in the Relevant Cellular Context
If you are studying cell cycle regulators, cell differentiation, or are developing anti-cancer compounds against cell cycle-related targets, Premo™ FUCCI is a superbly sensitive and accurate sensor tool. The efficient transduction and transient expression allows you to conduct these experiments in the biologically relevant cell types.

Simple, Quick Cell Cycle Visualization
Simply add the ready-to-use Premo™ FUCCI cell cycle sensor to your cells in complete medium, incubate overnight, then visualize cell cycle progression in populations of cells using fluorescence microscopy, flow cytometry, or high content imaging instruments. Cells change from red in the G1 to yellow in the G1/S interphase and green in S, G2, and M phases, as fusions of emGFP and TagRFP coupled to two cell cycle-regulated proteins are expressed and degraded.

How It Works
Premo™ FUCCI is derived from the fluorescence ubiquitination cell cycle indicator (FUCCI), developed by Miyawaki and colleagues (Ref 1). FUCCI is based on two cell cycle-regulated proteins, geminin and Cdt1, fused to one green (emGFP) and red (TagRFP) fluorescent protein, respectively. As Cdt1 and geminin are present only during specific phases of the cell cycle, the fluorescent protein partner is similarly cell-cycle dependent. Ubiquitination by specific ubiquitin E3 ligases target the fusion constructs in the proteasome for degradation. E3 ligases display temporal regulation of activity, resulting in biphasic cycling of geminin and Cdt1 levels during the cell cycle. Geminin-GFP is degraded in the G1 phase; the presence of Cdt1-TagRFP is indicated by red fluorescence within nuclei. During the S, G2, and M phases, however, Cdt1 is degraded and only geminin-GFP remains, giving cells green-fluorescent nuclei. During the G1/S transition, when Cdt1 levels are decreasing and geminin levels are increasing, both proteins are present, giving a yellow-fluorescent nuclear signal. This dynamic color change (red to yellow to green) clearly displays progression through the cell cycle and division.

Powered by BacMam 2.0 Technology
BacMam technology is based on an insect virus (baculovirus) for efficient transduction and transient expression in mammalian cells. The Premo™ FUCCI cell cycle sensor uses BacMam 2.0 technology to enable cell cycle studies in essentially any cell type, from cell lines to stem cells by incorporation of a mammalian expression cassette for the FUCCI construct. Compared with BacMam 1.0 technology, BacMam 2.0 does not require the use of an enhancer and can be used in complete medium. There is no need to purify plasmid or worry about vector integrity and quality. No lipids, dye-loading chemicals, or other potentially harmful treatments are required. BacMam 2.0 incorporates elements that greatly enhance transduction efficiency and expression levels: a pseudotyped capsid protein for more efficient cell entry, an enhanced CMV promoter, and the Woodchuck hepatitis post-transcriptional regulatory element (WPRE).

Titratable Expression that Lasts Up to Two Weeks
The transient transgene expression lasts from about 5 days in transformed cell lines to up to two weeks in slowly dividing cells, such as some primary cell types; we have imaged terminally differentiated neurons more than three weeks after transduction. In addition, BacMam technology permits defined optimization because it gives you the ability to precisely titrate expression levels. Baculoviruses do not replicate in mammalian cells, nor are viral genes expressed, giving them an excellent safety profile and lack of cytopathic effects on mammalian cells.

Learn more about the Premo™ FUCCI Cell Cycle Sensor

Reference:

1. Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A. Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell. 2008 Feb 8;132(3):487-98. PubMed PMID: 18267078

Related products
We offer a range of BacMam-based reagents beyond CellLight® reagents, including the BacMam GFP Transduction Control that is ideal to test out the technology and optimize transduction conditions, Premo™ Biosensors, including Premo™ Autophagy Sensor, ion channel drug targets, pathway analysis kits, and more.

Learn more about these products and the BacMam technology

See other imaging tools and reagents

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use


规格

Color:Green, Red-Orange
Delivery Method:Baculovirus (BacMam System)
Detection Method:Fluorescent
Emission Class:Visible
Excitation Class:Visible
Excitation⁄Emission (nm):488⁄510 (Green), 555⁄584 (Red)
For Use With (Equipment):Flow Cytometer, Fluorescence Microscope, Fluorescent Imager, High Content Instrument, Tali® Image-Based Cytometer
Form:Liquid
Label or Dye:GFP (EmGFP), RFP (TagRFP)
Product Line:Premo™
Product Size:2 x 200 µL
Quantity:2 vials (200 µl each)
Sub-Cellular Localization:Nucleus
Technique:Fluorescence Intensity
Shipping Condition:Wet Ice


规格

Color:Green, Red-Orange
Delivery Method:Baculovirus (BacMam System)
Detection Method:Fluorescent
Emission Class:Visible
Excitation Class:Visible
Excitation⁄Emission (nm):488⁄510 (Green), 555⁄584 (Red)
For Use With (Equipment):Flow Cytometer, Fluorescence Microscope, Fluorescent Imager, High Content Instrument, Tali® Image-Based Cytometer
Form:Liquid
Label or Dye:GFP (EmGFP), RFP (TagRFP)
Product Line:Premo™
Product Size:2 x 200 µL
Quantity:2 vials (200 µl each)
Sub-Cellular Localization:Nucleus
Technique:Fluorescence Intensity
Shipping Condition:Wet Ice


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