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1
2018 - 05 - 18
5月18日消息,哈佛大学的创业者Rejuvenate Bio已经开始测试为犬类延长寿命的方法,如果这项技术获得成功,那么将在人类身上进行试验。公司的合创者,63岁的George Church称,如果在查理士王小猎犬身上进行的医学测试成果的话,他将在自己身上进行测试。       查理士王小猎犬通常有一种心脏上的小疾病,导致半数物种在10岁左右时会因病死去。研究人员的研究已经表明,改变单一生物体的基因能够使其寿命加倍,并且在苍蝇身上取得了成功。这项研究的目的是观察在保持物种年轻身体状态的同时延长物种寿命会带来什么样的结果。Church教授被视作 “基因编辑”领域的一位先驱者,而且也正致力于借助史前DNA复活猛犸象的研究。他的返老还童实验室将发表一份关于这项技术的报告,研究人员将对老龄化疾病(比如说心脏病和糖尿病等)相关的两种基因进行修改,从而延长啮齿动物的寿命。麻省理工学院《技术评论》去年6月获得的一份文件表明,该创业公司在塔夫茨陆军兽医学院对4只米格鲁猎犬进行了测试。虽然文件中关于这次医学实验的过程透漏的很少,但是麻省理工学院的《技术评论》杂志推测,这一过程可能需要抑制一种充当“总开关”的蛋白质。Church教授称:“我们已经在老鼠身上进行了一系列的实验,而且我们正在犬类身上进行一些实验,随后我们将在人类身上进行这项技术的实验。犬类不仅仅是接...
2
2019 - 03 - 08
1 月 28 日,国际学术期刊 Circulation Research 在线发表了中国科学院上海营养与健康研究所宗耕课题组与哈佛大学公共卫生学院营养系合作的最新研究成果 “Associations of Monounsaturated Fatty Acids from Plant and Animal Sources with Total and Cause-Specific Mortality in Two US Prospective Cohort Studies”。该研究发现较高的植物来源单不饱和脂肪酸(MUFA-Ps)摄入量与较低的总死亡率相关,而动物来源单不饱和脂肪酸(MUFA-As)的摄入则与较高死亡率相关。另外,用植物来源单不饱和脂肪酸替代饱和脂肪酸(SFAs)、精加工的碳水化合物或反式脂肪可以显著降低死亡风险。在全球范围内,心血管疾病(CVD)和癌症是造成过早死亡的最主要原因。目前,越来越多的研究表明采取健康的生活方式(如戒烟、增加体力活动和提高膳食质量等)能够有效降低过早死亡的风险。多个国家的膳食指南均强调了膳食脂肪的质量对预防慢性疾病的重要性,尤其提倡植物油及其他植物来源的脂肪摄入。例如,膳食中的多不饱和脂肪酸(PUFAs)主要来自植物,而现有的研究表明 PUFAs 能够降低心血管疾病风险和死亡率的关系。与之相比,饮食中的单不饱和脂肪酸只有一半来自植物油(尤其...
3
2019 - 07 - 05
据新华社电 美国疾病控制和预防中心研究团队发现,正在刚果(金)使用的两种试验性疗法对抗击当前的埃博拉疫情有效。7 月 9 日发表在英国《柳叶刀—传染病》杂志上的研究显示,在实验室中,抗病毒药物 Remdesivir,以及由三种抗体组成的抗埃博拉药物 ZMapp 可阻断新一轮埃博拉疫情的致病毒株在人类细胞中增长。确认抗病毒药物有效的关键之一在于找到此次疫情的致病毒株。论文第一作者、美疾控中心微生物学家劳拉 · 麦克马伦说,目前在刚果(金)试用的疗法均针对以前疫情中获取的埃博拉毒株,而埃博拉是一种核糖核酸(RNA)病毒,会不断变异,因此确定现有疗法是否对新毒株奏效至关重要。美疾控中心研究人员没有当前在刚果(金)传播的 “伊图里” 毒株样本,但他们从开源基因库中获得病毒序列数据,使用 “反向遗传学” 技术重组了毒株。这项技术有助于研究人员更多了解新毒株在埃博拉家族树中的位置,从而寻找更多潜在疗法,并测试各类新疗法的有效性,还可与未来的新毒株进行比对。研究中还发现,2014 年到 2016 年西非多国爆发埃博拉疫情期间开发的一种实验室病毒诊断方法可用于诊断 “伊图里” 毒株。(周舟)文章摘自:生物360
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产品名称:

Premo™ FUCCI Cell Cycle Sensor (BacMam 2.0)

上市日期: 2019-04-18
产品类别: INVITROGEN

规格

Color:Green, Red-Orange
Delivery Method:Baculovirus (BacMam System)
Detection Method:Fluorescent
Emission Class:Visible
Excitation Class:Visible
Excitation⁄Emission (nm):488⁄510 (Green), 555⁄584 (Red)
For Use With (Equipment):Flow Cytometer, Fluorescence Microscope, Fluorescent Imager, High Content Instrument, Tali® Image-Based Cytometer
Form:Liquid
Label or Dye:GFP (EmGFP), RFP (TagRFP)
Product Line:Premo™
Product Size:2 x 200 µL
Quantity:2 vials (200 µl each)
Sub-Cellular Localization:Nucleus
Technique:Fluorescence Intensity
Shipping Condition:Wet Ice


  • 产品描述
  • 产品功能
  • 产品参数

描述

Premo™ FUCCI Cell Cycle Sensor is a fluorescent, two-color sensor of cell cycle progression and division in live cells. It allows accurate and sensitive cell cycle analysis of individual cells or a population of cells by fluorescence microscopy, flow cytometry, or high-content imaging.

At 2 x 200 µL, this is a smaller pack size version (1/5 volume) of the popular full-size product and perfect for testing its performance in your system.

The Premo™ FUCCI Cell Cycle Sensor is delivered by highly efficient BacMam 2.0 technology, enabling cell cycle studies in essentially any cell type. Provided in a ready-to-use format—simply add, incubate, and image. Premo™ FUCCI affords highly efficient transient expression in cell lines, primary cells, and stem cells.

Premo™ FUCCI Cell Cycle Sensor is:

Accurate: Cell-cycle controlled expression of bright GFP and RFP indicators for live cell analysis of individual cells or populations
Highly Efficient: >90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Fast and Convenient: Simply add Premo™ FUCCI Cell Cycle Sensor to your cells in complete medium, incubate overnight, and analyze
Safe: BacMam reagents are non-integrating and non-replicating in mammalian cells, lack observable cytopathic effects, and are suitable for biosafety level (BSL) 1 handling

Study Cell Cycle in the Relevant Cellular Context
If you are studying cell cycle regulators, cell differentiation, or are developing anti-cancer compounds against cell cycle-related targets, Premo™ FUCCI is a superbly sensitive and accurate sensor tool. The efficient transduction and transient expression allows you to conduct these experiments in the biologically relevant cell types.

Simple, Quick Cell Cycle Visualization
Simply add the ready-to-use Premo™ FUCCI cell cycle sensor to your cells in complete medium, incubate overnight, then visualize cell cycle progression in populations of cells using fluorescence microscopy, flow cytometry, or high content imaging instruments. Cells change from red in the G1 to yellow in the G1/S interphase and green in S, G2, and M phases, as fusions of emGFP and TagRFP coupled to two cell cycle-regulated proteins are expressed and degraded.

How It Works
Premo™ FUCCI is derived from the fluorescence ubiquitination cell cycle indicator (FUCCI), developed by Miyawaki and colleagues (Ref 1). FUCCI is based on two cell cycle-regulated proteins, geminin and Cdt1, fused to one green (emGFP) and red (TagRFP) fluorescent protein, respectively. As Cdt1 and geminin are present only during specific phases of the cell cycle, the fluorescent protein partner is similarly cell-cycle dependent. Ubiquitination by specific ubiquitin E3 ligases target the fusion constructs in the proteasome for degradation. E3 ligases display temporal regulation of activity, resulting in biphasic cycling of geminin and Cdt1 levels during the cell cycle. Geminin-GFP is degraded in the G1 phase; the presence of Cdt1-TagRFP is indicated by red fluorescence within nuclei. During the S, G2, and M phases, however, Cdt1 is degraded and only geminin-GFP remains, giving cells green-fluorescent nuclei. During the G1/S transition, when Cdt1 levels are decreasing and geminin levels are increasing, both proteins are present, giving a yellow-fluorescent nuclear signal. This dynamic color change (red to yellow to green) clearly displays progression through the cell cycle and division.

Powered by BacMam 2.0 Technology
BacMam technology is based on an insect virus (baculovirus) for efficient transduction and transient expression in mammalian cells. The Premo™ FUCCI cell cycle sensor uses BacMam 2.0 technology to enable cell cycle studies in essentially any cell type, from cell lines to stem cells by incorporation of a mammalian expression cassette for the FUCCI construct. Compared with BacMam 1.0 technology, BacMam 2.0 does not require the use of an enhancer and can be used in complete medium. There is no need to purify plasmid or worry about vector integrity and quality. No lipids, dye-loading chemicals, or other potentially harmful treatments are required. BacMam 2.0 incorporates elements that greatly enhance transduction efficiency and expression levels: a pseudotyped capsid protein for more efficient cell entry, an enhanced CMV promoter, and the Woodchuck hepatitis post-transcriptional regulatory element (WPRE).

Titratable Expression that Lasts Up to Two Weeks
The transient transgene expression lasts from about 5 days in transformed cell lines to up to two weeks in slowly dividing cells, such as some primary cell types; we have imaged terminally differentiated neurons more than three weeks after transduction. In addition, BacMam technology permits defined optimization because it gives you the ability to precisely titrate expression levels. Baculoviruses do not replicate in mammalian cells, nor are viral genes expressed, giving them an excellent safety profile and lack of cytopathic effects on mammalian cells.

Learn more about the Premo™ FUCCI Cell Cycle Sensor

Reference:

1. Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A. Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell. 2008 Feb 8;132(3):487-98. PubMed PMID: 18267078

Related products
We offer a range of BacMam-based reagents beyond CellLight® reagents, including the BacMam GFP Transduction Control that is ideal to test out the technology and optimize transduction conditions, Premo™ Biosensors, including Premo™ Autophagy Sensor, ion channel drug targets, pathway analysis kits, and more.

Learn more about these products and the BacMam technology

See other imaging tools and reagents

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use


规格

Color:Green, Red-Orange
Delivery Method:Baculovirus (BacMam System)
Detection Method:Fluorescent
Emission Class:Visible
Excitation Class:Visible
Excitation⁄Emission (nm):488⁄510 (Green), 555⁄584 (Red)
For Use With (Equipment):Flow Cytometer, Fluorescence Microscope, Fluorescent Imager, High Content Instrument, Tali® Image-Based Cytometer
Form:Liquid
Label or Dye:GFP (EmGFP), RFP (TagRFP)
Product Line:Premo™
Product Size:2 x 200 µL
Quantity:2 vials (200 µl each)
Sub-Cellular Localization:Nucleus
Technique:Fluorescence Intensity
Shipping Condition:Wet Ice


规格

Color:Green, Red-Orange
Delivery Method:Baculovirus (BacMam System)
Detection Method:Fluorescent
Emission Class:Visible
Excitation Class:Visible
Excitation⁄Emission (nm):488⁄510 (Green), 555⁄584 (Red)
For Use With (Equipment):Flow Cytometer, Fluorescence Microscope, Fluorescent Imager, High Content Instrument, Tali® Image-Based Cytometer
Form:Liquid
Label or Dye:GFP (EmGFP), RFP (TagRFP)
Product Line:Premo™
Product Size:2 x 200 µL
Quantity:2 vials (200 µl each)
Sub-Cellular Localization:Nucleus
Technique:Fluorescence Intensity
Shipping Condition:Wet Ice


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