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1
2019 - 02 - 28
据新华社电 瑞典哥德堡大学一项新研究表明,中年时期积极进行体力和脑力活动,有助降低罹患老年痴呆症的风险。哥德堡大学研究人员近日发表在美国《神经学》杂志上的一篇论文说,他们对 800 名瑞典女性进行了长达 44 年的跟踪调查,这些女性刚参与研究时平均年龄为 47 岁,并被按照参与脑力和体力活动的情况打分及分组。该研究中,脑力活动包括阅读和写作等智力活动、听音乐会和参加合唱团等艺术活动以及做针线活等手工活动。44% 的参与者被划分到脑力活动水平较低的低分组;56% 的人被划分到高分组。对体力活动,则分为活跃组和不活跃组。每周进行 4 小时以上步行、骑单车等低强度运动,或每周有规律地进行高强度运动如跑步、游泳等,或参与竞技运动,被划分到活跃组。按此标准,17% 的参与者被视为不活跃;82% 的人被视为活跃。研究发现,在排除了高血压、抽烟和糖尿病等风险因素后,脑力活动高分组女性比低分组女性罹患阿尔茨海默病的风险低 46%,罹患老年痴呆症的总体风险低 34%;而体力活动活跃组的女性比不活跃组女性罹患血管性痴呆症的风险低 52%,罹患混合型痴呆症的风险低 56%。摘自:生物360来源:中国科学报
2
2019 - 07 - 12
大脑每天接收的来自客观世界的感觉信息纷繁复杂,大脑对这些刺激进行分类后,人们才有感知判断。那么大脑是如何开展这项工作的呢?中科院脑科学与智能技术卓越创新中心(中科院神经科学研究所)研究员徐宁龙团队揭示了其中的奥秘,解析了大脑对感觉信息进行范畴化的皮层神经元群体运算机制。相关成果日前在线发表于《神经元》。大脑每天接收海量的来自客观世界的感觉信息,能够形成的概念和采取的行动却数目有限,为形成有意义的认知来指导行为,大脑需要对这些信息进行高效的组织管理,其中最基本的过程就是范畴化——简言之,就是分类。神经科学家本世纪初开启了信息分类和感知觉范畴化神经机制研究的新领域,但以往研究侧重神经运算的结果,对于感觉信息怎样被转化为离散的类别信息这一神经运算过程,并没有明确答案。本研究中,研究人员在小鼠中建立了一个基于听觉的分类抉择行为范式,结合活体双光子成像技术,解析了对感觉信息进行范畴化的皮层神经元群体运算机制。徐宁龙介绍,听到声音,经过训练的小鼠大脑中的一类神经元表现出对声音类别的特异性反应,将不同频率的纯音归类到 “高音” 或“低音”范畴。在高低音临界点的分类中,小鼠会“纠结”。这时大脑中的另一类神经元,表现出对类别边界频率声音的“选择性反应”,协助小鼠进行判断和分类。如果小鼠没有被要求完成分类任务,这类神经元选择性反应则“难觅踪迹”。研究表明,在听觉皮层中存在范畴抉择相关的单细胞反应,听觉...
3
2020 - 02 - 14
2020年2月12日讯 /生物谷BIOON /--弗朗西斯克里克研究所(Francis Crick Institute)的研究人员发现了一种控制组织结构的关键机制,这种机制可能有助于识别使癌细胞更难扩散的药物。这项发表在《Nature Materials》上的研究解释了导致组织结构变化的机制。这些研究利用实验生物学和计算生物学,确定了细胞之间的碰撞如何帮助创建不同的组织结构。其中一些结构有助于癌症扩散,作者还发现了抑制这一过程的药物。图片来源:Nature Materials体内的所有组织都含有维持其结构的蛋白质支架。这种支架会随着人的衰老而退化,导致出现皱纹等衰老迹象。癌细胞可以破坏支架的形状,从而创造出一个组织结构,并通过各种途径从肿瘤进入周围组织。癌细胞利用这些超级高速公路沿着它们扩散到新的区域。然而,虽然我们知道这些超级高速公路与癌症的发展有关,但对于控制这些组织结构形成的机制却知之甚少。克里克肿瘤细胞生物学实验室的研究科学家Danielle Park说:'这些超级高速公路为癌细胞走出肿瘤并在组织中更广泛地扩散提供了道路,这可能会给病人带来灾难性的后果。通过更多地了解这种结构是如何形成的,我们就能找到阻止它的方法,并对癌细胞的扩散设置障碍。'通过将实验室实验的观察结果与该团队开发的新计算机模型的结果相结合,研究人员发现了组织支架的形状如何受到其与帮助构建支架...
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产品名称:

Premo™ FUCCI Cell Cycle Sensor (BacMam 2.0)

上市日期: 2019-04-18
产品类别: INVITROGEN

规格

Color:Green, Red-Orange
Delivery Method:Baculovirus (BacMam System)
Detection Method:Fluorescent
Emission Class:Visible
Excitation Class:Visible
Excitation⁄Emission (nm):488⁄510 (Green), 555⁄584 (Red)
For Use With (Equipment):Flow Cytometer, Fluorescence Microscope, Fluorescent Imager, High Content Instrument, Tali® Image-Based Cytometer
Form:Liquid
Label or Dye:GFP (EmGFP), RFP (TagRFP)
Product Line:Premo™
Product Size:2 x 200 µL
Quantity:2 vials (200 µl each)
Sub-Cellular Localization:Nucleus
Technique:Fluorescence Intensity
Shipping Condition:Wet Ice


  • 产品描述
  • 产品功能
  • 产品参数

描述

Premo™ FUCCI Cell Cycle Sensor is a fluorescent, two-color sensor of cell cycle progression and division in live cells. It allows accurate and sensitive cell cycle analysis of individual cells or a population of cells by fluorescence microscopy, flow cytometry, or high-content imaging.

At 2 x 200 µL, this is a smaller pack size version (1/5 volume) of the popular full-size product and perfect for testing its performance in your system.

The Premo™ FUCCI Cell Cycle Sensor is delivered by highly efficient BacMam 2.0 technology, enabling cell cycle studies in essentially any cell type. Provided in a ready-to-use format—simply add, incubate, and image. Premo™ FUCCI affords highly efficient transient expression in cell lines, primary cells, and stem cells.

Premo™ FUCCI Cell Cycle Sensor is:

Accurate: Cell-cycle controlled expression of bright GFP and RFP indicators for live cell analysis of individual cells or populations
Highly Efficient: >90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Fast and Convenient: Simply add Premo™ FUCCI Cell Cycle Sensor to your cells in complete medium, incubate overnight, and analyze
Safe: BacMam reagents are non-integrating and non-replicating in mammalian cells, lack observable cytopathic effects, and are suitable for biosafety level (BSL) 1 handling

Study Cell Cycle in the Relevant Cellular Context
If you are studying cell cycle regulators, cell differentiation, or are developing anti-cancer compounds against cell cycle-related targets, Premo™ FUCCI is a superbly sensitive and accurate sensor tool. The efficient transduction and transient expression allows you to conduct these experiments in the biologically relevant cell types.

Simple, Quick Cell Cycle Visualization
Simply add the ready-to-use Premo™ FUCCI cell cycle sensor to your cells in complete medium, incubate overnight, then visualize cell cycle progression in populations of cells using fluorescence microscopy, flow cytometry, or high content imaging instruments. Cells change from red in the G1 to yellow in the G1/S interphase and green in S, G2, and M phases, as fusions of emGFP and TagRFP coupled to two cell cycle-regulated proteins are expressed and degraded.

How It Works
Premo™ FUCCI is derived from the fluorescence ubiquitination cell cycle indicator (FUCCI), developed by Miyawaki and colleagues (Ref 1). FUCCI is based on two cell cycle-regulated proteins, geminin and Cdt1, fused to one green (emGFP) and red (TagRFP) fluorescent protein, respectively. As Cdt1 and geminin are present only during specific phases of the cell cycle, the fluorescent protein partner is similarly cell-cycle dependent. Ubiquitination by specific ubiquitin E3 ligases target the fusion constructs in the proteasome for degradation. E3 ligases display temporal regulation of activity, resulting in biphasic cycling of geminin and Cdt1 levels during the cell cycle. Geminin-GFP is degraded in the G1 phase; the presence of Cdt1-TagRFP is indicated by red fluorescence within nuclei. During the S, G2, and M phases, however, Cdt1 is degraded and only geminin-GFP remains, giving cells green-fluorescent nuclei. During the G1/S transition, when Cdt1 levels are decreasing and geminin levels are increasing, both proteins are present, giving a yellow-fluorescent nuclear signal. This dynamic color change (red to yellow to green) clearly displays progression through the cell cycle and division.

Powered by BacMam 2.0 Technology
BacMam technology is based on an insect virus (baculovirus) for efficient transduction and transient expression in mammalian cells. The Premo™ FUCCI cell cycle sensor uses BacMam 2.0 technology to enable cell cycle studies in essentially any cell type, from cell lines to stem cells by incorporation of a mammalian expression cassette for the FUCCI construct. Compared with BacMam 1.0 technology, BacMam 2.0 does not require the use of an enhancer and can be used in complete medium. There is no need to purify plasmid or worry about vector integrity and quality. No lipids, dye-loading chemicals, or other potentially harmful treatments are required. BacMam 2.0 incorporates elements that greatly enhance transduction efficiency and expression levels: a pseudotyped capsid protein for more efficient cell entry, an enhanced CMV promoter, and the Woodchuck hepatitis post-transcriptional regulatory element (WPRE).

Titratable Expression that Lasts Up to Two Weeks
The transient transgene expression lasts from about 5 days in transformed cell lines to up to two weeks in slowly dividing cells, such as some primary cell types; we have imaged terminally differentiated neurons more than three weeks after transduction. In addition, BacMam technology permits defined optimization because it gives you the ability to precisely titrate expression levels. Baculoviruses do not replicate in mammalian cells, nor are viral genes expressed, giving them an excellent safety profile and lack of cytopathic effects on mammalian cells.

Learn more about the Premo™ FUCCI Cell Cycle Sensor

Reference:

1. Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A. Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell. 2008 Feb 8;132(3):487-98. PubMed PMID: 18267078

Related products
We offer a range of BacMam-based reagents beyond CellLight® reagents, including the BacMam GFP Transduction Control that is ideal to test out the technology and optimize transduction conditions, Premo™ Biosensors, including Premo™ Autophagy Sensor, ion channel drug targets, pathway analysis kits, and more.

Learn more about these products and the BacMam technology

See other imaging tools and reagents

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use


规格

Color:Green, Red-Orange
Delivery Method:Baculovirus (BacMam System)
Detection Method:Fluorescent
Emission Class:Visible
Excitation Class:Visible
Excitation⁄Emission (nm):488⁄510 (Green), 555⁄584 (Red)
For Use With (Equipment):Flow Cytometer, Fluorescence Microscope, Fluorescent Imager, High Content Instrument, Tali® Image-Based Cytometer
Form:Liquid
Label or Dye:GFP (EmGFP), RFP (TagRFP)
Product Line:Premo™
Product Size:2 x 200 µL
Quantity:2 vials (200 µl each)
Sub-Cellular Localization:Nucleus
Technique:Fluorescence Intensity
Shipping Condition:Wet Ice


规格

Color:Green, Red-Orange
Delivery Method:Baculovirus (BacMam System)
Detection Method:Fluorescent
Emission Class:Visible
Excitation Class:Visible
Excitation⁄Emission (nm):488⁄510 (Green), 555⁄584 (Red)
For Use With (Equipment):Flow Cytometer, Fluorescence Microscope, Fluorescent Imager, High Content Instrument, Tali® Image-Based Cytometer
Form:Liquid
Label or Dye:GFP (EmGFP), RFP (TagRFP)
Product Line:Premo™
Product Size:2 x 200 µL
Quantity:2 vials (200 µl each)
Sub-Cellular Localization:Nucleus
Technique:Fluorescence Intensity
Shipping Condition:Wet Ice


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