产品中心 Products
最新新闻 / News More
1
2022 - 03 - 17
来源:生物谷 2021-03-14 00:052021年3月12日讯/生物谷BIOON/--汽水是一种备受欢迎的饮料,在全世界被广泛消费。食品制造商为了丰富汽水的口味,开发了各种汽水饮品。其中不乏含糖饮料。我们知道,食品中的添加糖会给健康带来负面影响,比如增加心血管疾病、肥胖和糖尿病风险等。那么,饮用含糖汽水对乳腺癌患者的影响如何呢?近日,来自美国布法罗大学的一项新研究表明,乳腺癌患者经常饮用含糖汽水会增加全死亡的风险,尤其是因乳腺癌死亡的风险。与从不或很少喝含糖汽水的女性相比,每周喝五次或以上含糖汽水的女性其全因死亡率要高出62%,而死于乳腺癌的可能性要高出85%。相关研究结果于3月2日发表在美国癌症研究协会的Cancer Epidemiology Biomarkers & Prevention期刊上,标题为Sugar-Sweetened Soda Consumption and Total and Breast Cancer Mortality: The Western New York Exposures and Breast Cancer (WEB) Study。该研究的第一作者、布法罗大学公共卫生和健康职业学院流行病学和环境卫生系的博士生Nadia Koyratty说,关于汽水和乳腺癌的研究是非常新颖的话题。因为乳腺癌非常普遍,关于生活方式选择的建议对乳腺癌幸存者...
2
2019 - 09 - 05
2016年《消失的微生物》一书问世,肠道微生态研究走入大众视野,目前全球范围内针对微生物与肥胖、哮喘、过敏、糖尿病、肿瘤、精神类疾病的研究正逐步开展。一、肠道微生物概况人体是由自身细胞及共生的大量微生物细胞所共同组成的复杂共生生命体。目前研究认为人体肠道内聚集的微生物数目可达1012~1014个,是人体自身细胞数目的10倍,微生物包含的基因数目超过987万个,人类自身基因数只有2万个左右,是人体自身基因数目的几百倍,这些后天获得的共生菌群及其所携带的遗传及分子信息即可称为微生物组。(一)人体肠道微生物是如何建立的?有研究表明,人体在出生成长过程中,主要在两个重要的环节、以两种不同的方式实现对肠道菌群的塑造。第一个环节是在分娩过程,婴儿会从母亲产道获得健康的肠道菌群,剖腹产和孕期抗生素则会改变正常婴儿菌群的建立。另一个环节则是出生后的哺乳及断奶后的膳食干预。在女性乳腺中存在着乳酸菌等系列微生物,这些微生物在哺乳过程中进入并定植于婴儿肠道。随着哺乳期的结束,日趋丰富的膳食外源营养物质则长期调节着人体肠道微生物中的的变化。(二)肠道微生物对人体健康有何影响?肠道菌群与人体互利共生,依靠人体没有消化的食物残渣和肠道分泌的黏液和脱落的死细胞作为营养来维持很高的种群水平。活跃生长的肠道菌群会产生大量的活性物质,同时包括短链脂肪酸、维生素和抗氧化等有益健康的产物,以及神经毒素、致癌物质和免疫毒素...
3
2019 - 08 - 30
最近,《自然》杂志报道了不少关于癌症的研究。上周,学术经纬团队分享了一则癌症研究的重要突破——通过荧光标记,科学家们能够看清癌细胞如何“腐蚀”周边的健康细胞,发生转移。今天,我们又读到了一篇关于癌症转移的最新论文,它深刻揭示了癌症转移的复杂性,并提醒我们,在攻克癌症的道路上,我们还有很长的路要走……先来聊聊癌症。我们知道,癌症的发展就好像是一个熊孩子长大一样,会分成不同的阶段。在早期,癌细胞会疯狂生长,侵入和传播(invasion and dissemination)到组织内,在身体里形成肿瘤。到了晚期,癌细胞则会发生转移(metastasis),跑到身体的其他器官里落地生根,形成新的病灶。现在的医学知识告诉我们,一旦癌症发生了转移,患者的生命就岌岌可危。可是,癌细胞在一个地方长得好好的,它为啥会发生转移呢?过去的很多科学家相信,这与一种叫做E-cadherin(E-钙粘蛋白)的蛋白质有关。从功能上看,它负责把不同的细胞“粘”在一起。如果癌细胞能够减少这种钙粘蛋白的表达,就会变得更有“活动性”,也更容易发生转移。不过,科学家们也观察到了一些例外——在一种叫做“乳腺导管癌”的癌症中,发生转移的癌细胞,里头的E-钙粘蛋白含量可不少。这是为啥呢?有科学家解释说,这是因为癌细胞慢慢地又把自己“粘”回到了一起。这听起来是个合理的解释,但没有让所有人感到信服。为了寻找其他原因,来自美国知名的约...
联系我们
北京照生行仪器设备有限公司
销售热线:010-62929079
公司总机:010-62929079
图文传真:010-62929277
总部地址:北京市海淀区西二旗西路热力公司院内泰禾文化园二层
产品名称:

Premo™ FUCCI Cell Cycle Sensor (BacMam 2.0)

上市日期: 2019-04-18
产品类别: INVITROGEN

规格

Color:Green, Red-Orange
Delivery Method:Baculovirus (BacMam System)
Detection Method:Fluorescent
Emission Class:Visible
Excitation Class:Visible
Excitation⁄Emission (nm):488⁄510 (Green), 555⁄584 (Red)
For Use With (Equipment):Flow Cytometer, Fluorescence Microscope, Fluorescent Imager, High Content Instrument, Tali® Image-Based Cytometer
Form:Liquid
Label or Dye:GFP (EmGFP), RFP (TagRFP)
Product Line:Premo™
Product Size:2 x 200 µL
Quantity:2 vials (200 µl each)
Sub-Cellular Localization:Nucleus
Technique:Fluorescence Intensity
Shipping Condition:Wet Ice


  • 产品描述
  • 产品功能
  • 产品参数

描述

Premo™ FUCCI Cell Cycle Sensor is a fluorescent, two-color sensor of cell cycle progression and division in live cells. It allows accurate and sensitive cell cycle analysis of individual cells or a population of cells by fluorescence microscopy, flow cytometry, or high-content imaging.

At 2 x 200 µL, this is a smaller pack size version (1/5 volume) of the popular full-size product and perfect for testing its performance in your system.

The Premo™ FUCCI Cell Cycle Sensor is delivered by highly efficient BacMam 2.0 technology, enabling cell cycle studies in essentially any cell type. Provided in a ready-to-use format—simply add, incubate, and image. Premo™ FUCCI affords highly efficient transient expression in cell lines, primary cells, and stem cells.

Premo™ FUCCI Cell Cycle Sensor is:

Accurate: Cell-cycle controlled expression of bright GFP and RFP indicators for live cell analysis of individual cells or populations
Highly Efficient: >90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Fast and Convenient: Simply add Premo™ FUCCI Cell Cycle Sensor to your cells in complete medium, incubate overnight, and analyze
Safe: BacMam reagents are non-integrating and non-replicating in mammalian cells, lack observable cytopathic effects, and are suitable for biosafety level (BSL) 1 handling

Study Cell Cycle in the Relevant Cellular Context
If you are studying cell cycle regulators, cell differentiation, or are developing anti-cancer compounds against cell cycle-related targets, Premo™ FUCCI is a superbly sensitive and accurate sensor tool. The efficient transduction and transient expression allows you to conduct these experiments in the biologically relevant cell types.

Simple, Quick Cell Cycle Visualization
Simply add the ready-to-use Premo™ FUCCI cell cycle sensor to your cells in complete medium, incubate overnight, then visualize cell cycle progression in populations of cells using fluorescence microscopy, flow cytometry, or high content imaging instruments. Cells change from red in the G1 to yellow in the G1/S interphase and green in S, G2, and M phases, as fusions of emGFP and TagRFP coupled to two cell cycle-regulated proteins are expressed and degraded.

How It Works
Premo™ FUCCI is derived from the fluorescence ubiquitination cell cycle indicator (FUCCI), developed by Miyawaki and colleagues (Ref 1). FUCCI is based on two cell cycle-regulated proteins, geminin and Cdt1, fused to one green (emGFP) and red (TagRFP) fluorescent protein, respectively. As Cdt1 and geminin are present only during specific phases of the cell cycle, the fluorescent protein partner is similarly cell-cycle dependent. Ubiquitination by specific ubiquitin E3 ligases target the fusion constructs in the proteasome for degradation. E3 ligases display temporal regulation of activity, resulting in biphasic cycling of geminin and Cdt1 levels during the cell cycle. Geminin-GFP is degraded in the G1 phase; the presence of Cdt1-TagRFP is indicated by red fluorescence within nuclei. During the S, G2, and M phases, however, Cdt1 is degraded and only geminin-GFP remains, giving cells green-fluorescent nuclei. During the G1/S transition, when Cdt1 levels are decreasing and geminin levels are increasing, both proteins are present, giving a yellow-fluorescent nuclear signal. This dynamic color change (red to yellow to green) clearly displays progression through the cell cycle and division.

Powered by BacMam 2.0 Technology
BacMam technology is based on an insect virus (baculovirus) for efficient transduction and transient expression in mammalian cells. The Premo™ FUCCI cell cycle sensor uses BacMam 2.0 technology to enable cell cycle studies in essentially any cell type, from cell lines to stem cells by incorporation of a mammalian expression cassette for the FUCCI construct. Compared with BacMam 1.0 technology, BacMam 2.0 does not require the use of an enhancer and can be used in complete medium. There is no need to purify plasmid or worry about vector integrity and quality. No lipids, dye-loading chemicals, or other potentially harmful treatments are required. BacMam 2.0 incorporates elements that greatly enhance transduction efficiency and expression levels: a pseudotyped capsid protein for more efficient cell entry, an enhanced CMV promoter, and the Woodchuck hepatitis post-transcriptional regulatory element (WPRE).

Titratable Expression that Lasts Up to Two Weeks
The transient transgene expression lasts from about 5 days in transformed cell lines to up to two weeks in slowly dividing cells, such as some primary cell types; we have imaged terminally differentiated neurons more than three weeks after transduction. In addition, BacMam technology permits defined optimization because it gives you the ability to precisely titrate expression levels. Baculoviruses do not replicate in mammalian cells, nor are viral genes expressed, giving them an excellent safety profile and lack of cytopathic effects on mammalian cells.

Learn more about the Premo™ FUCCI Cell Cycle Sensor

Reference:

1. Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A. Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell. 2008 Feb 8;132(3):487-98. PubMed PMID: 18267078

Related products
We offer a range of BacMam-based reagents beyond CellLight® reagents, including the BacMam GFP Transduction Control that is ideal to test out the technology and optimize transduction conditions, Premo™ Biosensors, including Premo™ Autophagy Sensor, ion channel drug targets, pathway analysis kits, and more.

Learn more about these products and the BacMam technology

See other imaging tools and reagents

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use


规格

Color:Green, Red-Orange
Delivery Method:Baculovirus (BacMam System)
Detection Method:Fluorescent
Emission Class:Visible
Excitation Class:Visible
Excitation⁄Emission (nm):488⁄510 (Green), 555⁄584 (Red)
For Use With (Equipment):Flow Cytometer, Fluorescence Microscope, Fluorescent Imager, High Content Instrument, Tali® Image-Based Cytometer
Form:Liquid
Label or Dye:GFP (EmGFP), RFP (TagRFP)
Product Line:Premo™
Product Size:2 x 200 µL
Quantity:2 vials (200 µl each)
Sub-Cellular Localization:Nucleus
Technique:Fluorescence Intensity
Shipping Condition:Wet Ice


规格

Color:Green, Red-Orange
Delivery Method:Baculovirus (BacMam System)
Detection Method:Fluorescent
Emission Class:Visible
Excitation Class:Visible
Excitation⁄Emission (nm):488⁄510 (Green), 555⁄584 (Red)
For Use With (Equipment):Flow Cytometer, Fluorescence Microscope, Fluorescent Imager, High Content Instrument, Tali® Image-Based Cytometer
Form:Liquid
Label or Dye:GFP (EmGFP), RFP (TagRFP)
Product Line:Premo™
Product Size:2 x 200 µL
Quantity:2 vials (200 µl each)
Sub-Cellular Localization:Nucleus
Technique:Fluorescence Intensity
Shipping Condition:Wet Ice


推荐新闻
地址:中国·北京·海淀区-西二旗西路热力公司院内泰禾文化园二层
电话:010-62929079
Copyright@ 2015-2016 北京照生行仪器设备有限公司
传真:+86 0755-2788 8009
邮编:330520