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1
2019 - 02 - 13
日前,《分子植物》(Molecular Plant)在线发表了中国农业科学院油料作物研究所王汉中院士团队最新成果。该团队在国际上首次成功克隆了农作物种子性状的第一个细胞质调控基因 orf188,并揭示了该基因调控油菜种子高含油量的作用机制。该成果为油菜高含油量育种提供了新途径,为育种过程中杂交母本的选择提供了理论支撑,对于农作物不同类型细胞质的应用具有重要的指导意义。含油量是油菜产油量最重要的构成因子之一,随着现代育种对含油量潜力的不断挖掘,提高品种的含油量越来越困难。该团队在油菜含油量遗传研究的基础上,从细胞质效应着手对含油量母体调控新机制研究取得突破,筛选到两个对含油量具有极显著细胞质正负效应的品种,鉴定出特异基因 orf188 控制细胞质的含油量正效应,过表达该基因可以大幅度提升含油量。研究表明,orf188 为 nap(nap-like)型油菜品种所特有基因,是一个新近进化形成的嵌合基因。该团队十余年来围绕油菜种子性状的母体调控进行系统研究,此次对细胞质基因调控种子含油量机理的揭示,是该团队继发现角果皮发育调控种子粒重和含油量之后发现的又一条母体调控新途径,进一步丰富和完善了种子性状的母体调控理论,也为其他农作物性状细胞质效应的解析提供了借鉴。相关论文信息:DOI:10.1016/j.molp.2019.01.012来源:中国科学报转载:生物360
2
2019 - 06 - 14
随着技术飞速发展、医学数据的持续扩增以及硬件设备的不断提升,人工智能和医疗的结合方式越来越多样化。目前AI在医疗领域中的落地的应用场景主要有医学影像、智能诊疗、智能导诊、智能语音、健康管理、病例分析、医院管理、新药研发和医疗机器人等,其中在医学影像中的应用最为广泛。一、影像医学发展现状医学影像是医生完成诊断的主要依据,通过对影像的分析和比较,从而完成有依据的诊断。但是在实际过程中,往往会存在以下问题:(1)影像学诊断人才资源紧缺。医疗机构普遍缺乏高水平的影像医师,在疾病诊断时往往会发生同病异影,异病同影等情况。(2)传统定性分析存在诊断误差。医生普遍擅长定性分析,很多微小的定量变化无法通过肉眼判断,很难做到定量分析。(3)医生阅片时间长。目前的影像呈现方式为数据和图像,而不是最有效的信息,很大程度上限制了医生的人工阅片速度。二、AI+医学影像助力疾病诊断通过引入人工智能可有效解决部分问题,目前人工智能在医学影像领域的应用方向主要以下几类:1. 影像设备的图像重建AI可以通过算法的图像映射技术,将采集的少量信号恢复出与全采样图像同样质量的图像,而且使用图像重建技术,可以由低剂量的CT和PET图像重建得到高剂量质量图像。这样在满足临床诊断需求的同时,还能够降低辐射的风险。2. 智能辅助诊断疾病(1)智能辅助诊断肺部疾病国内应用AI+CT影像最为成熟的领域在肺结节的识别上。AI能够有效识...
3
2019 - 12 - 25
前瞻记忆是指个体记住未来要完成任务的能力。儿童进入学校后,前瞻记忆任务逐渐增多,对前瞻记忆能力的要求也逐渐增高。前瞻记忆任务成功的关键在于当个体看到线索时能主动提取并执行意图。前瞻记忆线索分为三种,时间线索(如周六晚上7点记得参加朋友的生日聚会)、事件线索(如路过超市记得买水果)和活动结束线索(如写完作业记得找家长签字)。三种线索的提取难度存在差异,这可能与其对注意资源的需求不同有关。较之正常发育的儿童,多动症儿童在日常活动中常有注意力不集中和健忘的表现,提示其前瞻记忆能力可能存在缺损。然而,对多动症儿童前瞻记忆功能的研究较少,更缺乏研究直接考察多动症儿童在三种线索条件下的前瞻记忆表现及其认知加工特点。因此,中国科学院心理研究所心理健康重点实验室神经心理和应用认知神经科学(NACN)实验室陈楚侨研究组与北医六院医生钱英、潍坊医学院教授王艳郁合作考察了多动症儿童在时间、事件和活动结束线索条件下的前瞻记忆表现。该研究招募了28名临床诊断的多动症儿童和28名年龄和性别匹配的正常发育儿童,采用钓鱼游戏任务(Yang, Chan, Shum, 2011)测查儿童在三种线索条件下的前瞻记忆功能。该任务采取经典的双任务范式,主任务要求儿童钓鱼,并同时关注前瞻记忆线索,在线索出现时儿童需要停止钓鱼并执行前瞻记忆任务。在时间线索条件下,儿童要记得每到整分钟就喂猫吃鱼;在事件线索条件下,儿童看到特殊条...
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总部地址:北京市海淀区西二旗西路热力公司院内泰禾文化园二层
产品名称:

EVOS™ FL Imaging System

上市日期: 2019-04-15
产品类别: EVOS

规格

Color:Transmitted Light
Data Output:BMP Files, JPG Files, PNG Files, TIFF Files
Detection Method:Fluorescent
Dimensions:36 cm (14 in) (W) x 58 cm (23 in) (H) x 47 cm (19 in) (D)
Display Interface:15-inch color monitor, Adjustable tilt
For Use With (Equipment):Fluorescence Microscope
Format:Specific Holder Attachments
Frequency:50⁄60 Hz
Input Voltage:100-240 VAC
Objectives:5 Objective Turret
Product Line:EVOS®
Product Size:1 each
Stage:Mechanical stage with X-Y axis fine-positioning controls
Weight:15.3 kg (33.7 lbs)


  • 产品描述
  • 产品功能
  • 产品参数

描述

The EVOS® FL Imaging System is a fully integrated, digital, inverted imaging system for four-color fluorescence and transmitted-light applications. It is powerful, yet easy-to-use and delivers high-definition images with exceptional convenience. The unique light cubes, sensitive camera, and precision-engineered optical system make the EVOS® FL system ideal for both demanding and routine fluorescence imaging applications.

The EVOS® FL Imaging System offers you these important advantages:

• Easy installation; no maintenance, assembly, alignment, or calibration
• For four-color fluorescence and transmitted light applications
• Five-position objective turret with front controls
• On-board software
• All-in-one design: digital camera, precision optics, LCD display, and USB storage

Fully Integrated Imaging System
The EVOS® FL system represents a new concept in transmitted light inverted microscopy. It is a fully integrated imaging system that combines precision optics, a 15' high-resolution LCD display, and a highly sensitive Sony ICX445 monochrome CCD camera (1280 x 960 pixel resolution, 1.3 Megapixels). Images are seamlessly acquired through the intuitive user interface using a mouse for easy control. Monochrome cameras are commonly used for high-performance fluorescence applications and provide the best sensitivity for detection of faint fluorescence signals. Color cameras, as on the EVOS® FL Color System, have lower fluorescence sensitivity but have the advantage of being able to differentiate structures by color in transmitted light (e.g., imaging stained tissue samples).

Compare cell imaging systems >

Versatile and Highly Configurable
The EVOS® FL system features an easily accessible 5-position objective turret and a 3-position condenser turret. The 4 fluorescence light cubes are lever-actioned for rapid and easy selection. The optical system can be configured to best meet your needs using our full range of high-quality objectives from 2x to 100x magnification. Lighting settings are automatically adjusted to match the objective magnification. The EVOS® FL system features a mechanical stage with X/Y-axis fine-positioning using control knobs conveniently located on the left side and the front of the stage. The stage accommodates EVOS® vessel holders that provide a perfect fit for most vessel types and sizes. Interchangeable stage plates accommodate most vessel types and sizes, including flasks, Petri dishes, multi-well plates, and slides.

Smart LED Illumination Technology
All EVOS® fluorescence imaging systems utilize our unique, proprietary LED light cubes. This world-leading light engine outputs remarkable intensity over a short light-path that delivers superior fluorophore excitation. Each light cube contains a precisely matched set of optical components to optimize the position, evenness, and intensity of the light beam. Digitally controlled LED light sources allow adjustment of illumination levels, dramatically improving control over photobleaching. Hard-coated filters give sharper edges and significantly higher transmission efficiencies than traditional soft-coated filters.

Integrated Software
The on-board computer makes the EVOS® FL system easy to run and use, and the intuitive, comprehensive software facilitates both image acquisition and analysis. Modules include time-lapse acquisition, manual-assist cell counting, and image review. Saved user settings work in conjunction with 'smart' controls to help increase efficiency and ease of use. Image data are saved to a USB flash drive for easy, portable data transfer or to a Windows-networked local server. Images can be saved in PNG, JPEG, BMP, and TIFF formats with a maximum captured image resolution of 1280 x 960 pixels.

These features make the EVOS® FL system ideal for both routine and demanding fluorescence imaging applications such as GFP-vector transfection efficiency analysis, routine cell and tissue culture, cell confluence determination, stem cell growth and differentiation, and developmental biology and tissue slice analyses. And the compact footprint makes it as easy to use the EVOS® FL system in a cell culture hood as on the bench.

Easy to Use and Reliable
The EVOS® FL Imaging System is plug-and-play. In addition to an exceptionally easy set-up and installation, it requires no warm-up or cool-down periods. The LED light source offers exceptional stability and durability—so you can turn the unit on and off whenever you need to image a sample. The environmentally safe mercury-free LED bulbs are rated for >50,000 hours (~17 years) of life, compared to 300 hours for a typical mercury bulb and 1,500 hours for a metal halide bulb. The long life and low energy consumption translate into significantly lower operating costs compared to instruments with conventional light sources. Finally, the advanced ergonomic design of the EVOS® systems removes the strain associated with conventional microscopes, enables shared viewing, and makes moving the units easy. For extended or precision use, the EVOS® arm rest provides comfortable support.

The EVOS® imaging systems are built from the ground up to maximize performance and optimize workflow. You will be astonished at how easy it is to operate and amazed at how extraordinary your images look on-screen.

Learn more about the EVOS® FL Imaging System
Explore the entire EVOS® line of imaging systems and accessories

System Highlights
Optics: infinity-corrected optical system, RMS-threaded objectives with 45 mm parfocal distance
Illumination: adjustable intensity LED (>50,000-hour life per light cube)
Light cubes (not included): broad selection of patented standard and specialty light cubes
Common light cubes include: DAPI (Ex 360 nm/Em 447 nm), GFP (Ex 470 nm/Em 525 nm), RFP (Ex 530 nm/Em 593 nm), Texas Red® (Ex 585 nm/Em 624), Cy5 (Ex 628 nm/Em 692 nm)
Contrast methods: fluorescence and transmitted light (brightfield & phase contrast)
Objective turret: 5-position, front-mounted control
Objectives (not included): wide selection of high-quality LWD and coverslip-corrected objectives available
Condenser: 3-position turret with brightfield and phase contrast annuli
Condenser working distance: 60 mm
Stage: mechanical 'glide' stage; X-Y axis fine-positioning controls: 28.3 mm (1.11') per rotation, 110 mm x 110 mm (4.3' x 4.3') range of motion; Z-axis focusing controls: 480 µm/rotation; interchangeable vessel holders are available for most common shapes and sizes
Focus mechanism: coaxial focus knobs with tension control
Course focus: 38 mm/rev
Fine focus: 0.2mm/rev, precision 0.002 mm
LCD display: 15-inch color, 1024 x 768 pixels, adjustable tilt
Camera: Sony ICX445 monochrome CCD, 1/3' 1280 x 960 pixels, 1.3 Megapixels
Image acquisition: onboard microprocessor, built-in software for image acquisition via mouse control
Captured images: 16-bit monochrome TIFF or PNG (12-bit dynamic range); 24-bit color TIFF or PNG; jpeg, bmp, 1280 x 960 pixels
Output ports: 3 USB and 1 DVI
Power supply: AC adapter with country-specific power cords; input 100-240 V, 50-60 Hz; output 12 VDC/4.15 A
Dimensions: operating height = 57.8 cm (22.75 in); storage/transport height = 32.4 cm (12.75 in); depth = 47.0 cm (18.5 in); width = 35.5 cm (14.0 in)
Weight: 15.3 kg (33.7 lbs) 


规格

Color:Transmitted Light
Data Output:BMP Files, JPG Files, PNG Files, TIFF Files
Detection Method:Fluorescent
Dimensions:36 cm (14 in) (W) x 58 cm (23 in) (H) x 47 cm (19 in) (D)
Display Interface:15-inch color monitor, Adjustable tilt
For Use With (Equipment):Fluorescence Microscope
Format:Specific Holder Attachments
Frequency:50⁄60 Hz
Input Voltage:100-240 VAC
Objectives:5 Objective Turret
Product Line:EVOS®
Product Size:1 each
Stage:Mechanical stage with X-Y axis fine-positioning controls
Weight:15.3 kg (33.7 lbs)


规格

Color:Transmitted Light
Data Output:BMP Files, JPG Files, PNG Files, TIFF Files
Detection Method:Fluorescent
Dimensions:36 cm (14 in) (W) x 58 cm (23 in) (H) x 47 cm (19 in) (D)
Display Interface:15-inch color monitor, Adjustable tilt
For Use With (Equipment):Fluorescence Microscope
Format:Specific Holder Attachments
Frequency:50⁄60 Hz
Input Voltage:100-240 VAC
Objectives:5 Objective Turret
Product Line:EVOS®
Product Size:1 each
Stage:Mechanical stage with X-Y axis fine-positioning controls
Weight:15.3 kg (33.7 lbs)


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