产品中心 Products
最新新闻 / News More
1
2018 - 07 - 05
doi.org/10.1038/s41586-018-0162-7基于20年的深入研究,得克萨斯大学西南医学中心(UT Southwestern)自噬研究中心的主任Beth Levine和团队已经明确:细胞自噬可以延长寿命、改善健康。他们以小鼠为模型发现,自噬水平提高的小鼠寿命更长、更健康。“具体来说,它们的寿命延长了约10%,且不太可能发生与年龄相关的自发性癌症、心脏病等疾病。”Beth Levine表示道。细胞自噬自噬是一种保护机制。当细胞内出现衰老的蛋白质、损坏的细胞器等废弃物时,自噬囊泡会将它们包裹并送至溶酶体中进行降解并得以循环利用,从而确保细胞本身的代谢需求和某些细胞器的更新。细胞自噬过程(图片来源:华威大学)20年前,Beth Levine和同事发现了自噬生物学过程中的一个关键基因beclin 1。他们还发现,自噬对于健康而言必不可少,例如预防神经衰退性疾病、对抗癌症和感染。2003年,Beth Levine团队证实,自噬关联的基因机制对于长寿突变线虫(long-lived mutant roundworms)的寿命延长至关重要。“从那以后,关于‘自噬是延长寿命的关键机制’的观点尤为火热。” Beth Levine解释道,“随着年龄增长,细胞的自噬能力会下降,这可能会导致衰老。”自噬延长寿命然而,一个关键问题仍然没有得到解答:增强自噬水平是否有益于哺乳动物的健康?换句...
2
2019 - 05 - 06
来源: 中国科学报年初,一部电视剧《都挺好》火遍全国,大结局时剧中一直让人恨得牙痒痒的 “作爹” 苏大强,因患上了阿尔茨海默氏症(AD,俗称老年痴呆症)而成功“洗白”,惹出了不少人的眼泪。阿尔茨海默氏症离我们并不遥远,由它引发的故事也是常有耳闻,大多伴随着无奈与辛酸。随着全球老龄化进程的加快,愈来愈多的阿尔茨海默氏症患者将给家属及社会带来沉重的负担。根据国际阿尔茨海默氏病协会的统计数据,2018 年全世界有 5000 万老人患有痴呆,每 3 秒钟,就有 1 位患者产生;中国是阿尔茨海默氏病的重灾区,估计有 1000 万患者。关于阿尔茨海默氏症发病机制和治疗方法的研究一直都是科研界关注的重点之一。近日,中国科学院昆明动物研究所姚永刚课题组在阿尔茨海默氏症的治疗研究上取得重要进展,该研究阐明了核受体过氧化物酶体α激动剂吉非罗齐和匹立尼酸的抗阿尔茨海默氏症分子机制。相关论文在线发表于《自噬》(Autophagy)杂志上。诱导自噬,清除 Aβ作为发生在老年前期与老年期的一种常见的神经退行性脑病,阿尔茨海默氏症是最常见的老年痴呆类型,其发病率随着年龄增长而显著增加。其临床表现为病人渐进性记忆丧失、认知能力下降并伴有焦虑等其他精神相关症状。对于阿尔茨海默氏症的发病机制,现有很多学说,其中影响最广泛的有β- 淀粉样蛋白(β-amyloid,Aβ)瀑布理论,认为 Aβ的生成与清除失衡是导致神经元变...
3
2019 - 09 - 05
2016年《消失的微生物》一书问世,肠道微生态研究走入大众视野,目前全球范围内针对微生物与肥胖、哮喘、过敏、糖尿病、肿瘤、精神类疾病的研究正逐步开展。一、肠道微生物概况人体是由自身细胞及共生的大量微生物细胞所共同组成的复杂共生生命体。目前研究认为人体肠道内聚集的微生物数目可达1012~1014个,是人体自身细胞数目的10倍,微生物包含的基因数目超过987万个,人类自身基因数只有2万个左右,是人体自身基因数目的几百倍,这些后天获得的共生菌群及其所携带的遗传及分子信息即可称为微生物组。(一)人体肠道微生物是如何建立的?有研究表明,人体在出生成长过程中,主要在两个重要的环节、以两种不同的方式实现对肠道菌群的塑造。第一个环节是在分娩过程,婴儿会从母亲产道获得健康的肠道菌群,剖腹产和孕期抗生素则会改变正常婴儿菌群的建立。另一个环节则是出生后的哺乳及断奶后的膳食干预。在女性乳腺中存在着乳酸菌等系列微生物,这些微生物在哺乳过程中进入并定植于婴儿肠道。随着哺乳期的结束,日趋丰富的膳食外源营养物质则长期调节着人体肠道微生物中的的变化。(二)肠道微生物对人体健康有何影响?肠道菌群与人体互利共生,依靠人体没有消化的食物残渣和肠道分泌的黏液和脱落的死细胞作为营养来维持很高的种群水平。活跃生长的肠道菌群会产生大量的活性物质,同时包括短链脂肪酸、维生素和抗氧化等有益健康的产物,以及神经毒素、致癌物质和免疫毒素...
联系我们
北京照生行仪器设备有限公司
销售热线:010-62929079
公司总机:010-62929079
图文传真:010-62929277
总部地址:北京市海淀区西二旗西路热力公司院内泰禾文化园二层
产品名称:

Click-iT™ EdU Pacific Blue™ Flow Cytometry Assay Kit

上市日期: 2019-04-17
产品类别: INVITROGEN

规格

Flow Cytometer Laser Lines:405
For Use With (Equipment):Flow Cytometer
Detection Method:Fluorescent
Format:Tube(s)
Excitation⁄Emission (nm):404⁄450
Label or Dye:Pacific Blue™
Number of Reactions:50
Product Line:Click-iT™, Pacific Blue™
Product Size:50 assays
Green Features:Less hazardous
Shipping Condition:Room Temperature


  • 产品描述
  • 产品功能
  • 产品参数

描述

The Click-iT® EdU Pacific Blue™ Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells as compared to traditional BrdU methods. Newly synthesized DNA is analyzed using the 405 nm laser of the flow cytometer.

• Accurate—superior results compared to BrdU assays
• Fast—results in as little as 90 minutes
• Economical—more assays per kit

View selection guide for all Click-iT™ EdU and Click-iT™ Plus EdU assays for flow cytometry.

An Advanced Method Giving You Results Superior to BrdU
The most accurate method of proliferation analysis is direct measurement of DNA synthesis. Originally, this was performed through incorporation of radioactive nucleosides, i.e., 3H-thymidine. This method was replaced by antibody-based detection of the nucleoside analog bromodeoxyuridine (BrdU). The Click-iT® EdU Flow Cytometry Assay Kits are novel alternatives to the BrdU assay. EdU (5-ethynyl-2´-deoxyuridine) is a thymidine analog which is incorporated into DNA during active DNA synthesis. Detection is based on click chemistry: a copper catalyzed covalent reaction between an azide and an alkyne. In this application, the alkyne is found in the ethynyl moiety of EdU, while the azide is coupled to Alexa Fluor ® 488, Alexa Fluor® 647, or Pacific Blue™ dyes. Standard flow cytometry methods are used for determining the percentage of S-phase cells in the population (Fig 1).

Mild Conditions Allow Use with Cell Cycle Dyes and Antibodies
The advantages of Click-iT® EdU labeling are readily evident while performing the assay (Fig 2). The small size of the dye azide allows for efficient detection of the incorporated EdU using mild conditions, while standard aldehyde-based fixation and detergent permeabilization are sufficient for the Click-iT® detection reagent to gain access to the DNA. This is in contrast to BrdU assays that require DNA denaturation (using HCl, heat, or digestion with DNase) to expose the BrdU so that it may be detected with an anti-BrdU antibody. Sample processing for the BrdU assay can result in signal alteration of the cell cycle distribution as well as destruction of antigen recognition sites when using the HCl method. In contrast, the easy-to-use EdU cell proliferation kit is compatible with cell cycle dyes. This EdU assay kit can also be multiplexed with antibodies against surface and intracellular markers.

Quick and Simple Protocol
The Click-iT® EdU protocol is based on the aldehyde fixation and detergent permeabilization steps for immunohistochemical antibody labeling, but EdU is compatible with other fixation/permeabilization agents including saponin and methanol. In just five steps you’ll be ready to analyze your cell proliferation data:

• Treat cells with EdUM
• Fix and permeabilize cells
• Detect S-phase cells with Click-iT® detection cocktail for 30 min
• Wash once
• Analyze

Get Accurate Results Economically
By increasing the number of assays per kit, the Click-IT® EdU Pacific Blue™ Flow Cytometry Assay Kit is less expensive than the traditional BrdU assays making them ideal for large experiments.

For Research Use Only. Not for use in diagnostic procedures.


规格

Flow Cytometer Laser Lines:405
For Use With (Equipment):Flow Cytometer
Detection Method:Fluorescent
Format:Tube(s)
Excitation⁄Emission (nm):404⁄450
Label or Dye:Pacific Blue™
Number of Reactions:50
Product Line:Click-iT™, Pacific Blue™
Product Size:50 assays
Green Features:Less hazardous
Shipping Condition:Room Temperature


规格

Flow Cytometer Laser Lines:405
For Use With (Equipment):Flow Cytometer
Detection Method:Fluorescent
Format:Tube(s)
Excitation⁄Emission (nm):404⁄450
Label or Dye:Pacific Blue™
Number of Reactions:50
Product Line:Click-iT™, Pacific Blue™
Product Size:50 assays
Green Features:Less hazardous
Shipping Condition:Room Temperature


地址:中国·北京·海淀区-西二旗西路热力公司院内泰禾文化园二层
电话:010-62929079
Copyright@ 2015-2016 北京照生行仪器设备有限公司
传真:+86 0755-2788 8009
邮编:330520