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1
2018 - 05 - 18
5月18日消息,哈佛大学的创业者Rejuvenate Bio已经开始测试为犬类延长寿命的方法,如果这项技术获得成功,那么将在人类身上进行试验。公司的合创者,63岁的George Church称,如果在查理士王小猎犬身上进行的医学测试成果的话,他将在自己身上进行测试。       查理士王小猎犬通常有一种心脏上的小疾病,导致半数物种在10岁左右时会因病死去。研究人员的研究已经表明,改变单一生物体的基因能够使其寿命加倍,并且在苍蝇身上取得了成功。这项研究的目的是观察在保持物种年轻身体状态的同时延长物种寿命会带来什么样的结果。Church教授被视作 “基因编辑”领域的一位先驱者,而且也正致力于借助史前DNA复活猛犸象的研究。他的返老还童实验室将发表一份关于这项技术的报告,研究人员将对老龄化疾病(比如说心脏病和糖尿病等)相关的两种基因进行修改,从而延长啮齿动物的寿命。麻省理工学院《技术评论》去年6月获得的一份文件表明,该创业公司在塔夫茨陆军兽医学院对4只米格鲁猎犬进行了测试。虽然文件中关于这次医学实验的过程透漏的很少,但是麻省理工学院的《技术评论》杂志推测,这一过程可能需要抑制一种充当“总开关”的蛋白质。Church教授称:“我们已经在老鼠身上进行了一系列的实验,而且我们正在犬类身上进行一些实验,随后我们将在人类身上进行这项技术的实验。犬类不仅仅是接...
2
2019 - 03 - 15
基因组的遗传信息得以表达,首先需要 RNA polymerase (RNAP) 以 DNA 为模板合成 RNA。基因转录不仅是基因表达第一步,还是基因表达的主要调控步骤。对 RNAP 分子机器结构、运行机理以及调控机制的研究能够回答基因表达调控的基础生物学问题。在转录起始阶段,细菌的 RNAP 与转录起始σ因子形成复合物,依次执行启动子双链 DNA 的识别、解链以及 RNA 起始合成等关键步骤。细菌 RNAP 通过与多个σ因子结合特异性调控基因转录,其中 Extra-Cytoplasmic Function(ECF)σ因子是细菌中种类最多的一类σ因子,它可以感受细菌胞内外环境变化,起始特异性的基因转录。ECF σ因子赋予细菌适应逆境的能力,对于致病菌的致病性和耐药性尤为重要。以结核分枝杆菌(Mycobacterium tuberculosis)为例,其 RNAP 分别与 10 种 ECF σ因子结合,通过识别特异启动子序列启动相应基因表达,多个 ECF σ因子与结核分枝杆菌的致病、侵染以及耐药直接相关。3 月 11 日,国际学术期刊《自然 - 通讯》(Nature communications)在线发表了中国科学院分子植物科学卓越创新中心 / 植物生理生态研究所 / 中科院合成生物学重点实验室张余研究组题为 Structure basis for transcription ini...
3
2019 - 07 - 19
近日,来自 9 个国家的 33 名微生物学家在《自然综述:微生物学》上发文表示,应该进一步将微生物融入主流气候变化研究和气候变化框架中,以应对人为造成的气候变化问题。他们警告说,“提醒人类注意,气候变化的影响将严重依赖微生物的反应,它们对于实现一个环境可持续发展的未来至关重要”。已知人类活动及其对气候和环境的影响关系到动植物灭绝和生物多样性损失,并且危及地球上的动植物生命。微生物也会对温室气体排放产生重要影响。尽管微生物丰富多样,但它们维护生态系统健康的作用,以及如何影响气候变化或被气候变化影响,却鲜为人知。澳大利亚新南威尔士大学的 Ricardo Cavicchioli 等人认为,微生物在气候变化生物学中扮演的核心角色及其全局重要性不应被低估,他们并且呼吁研究人员、机构和政府对微生物加剧或缓解人为气候变化影响的作用进行评估。研究人员在文中探讨了气候变化对微生物作用以及全球海陆生物群落的影响。他们还指出,从农业和传染病的角度出发,需要更深入地理解气候变化对微生物的影响,比如气候变化预计将提高部分人类病原体的抗生素耐药性。相关论文信息:https://doi.org/10.1038/s41579-019-0222-5
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产品名称:

Dynabeads™ CD45

上市日期: 2019-04-17
产品类别: INVITROGEN

Specifications

Capacity:Processes ~5x10^8 cells total
Cell Type:Leukocytes, Tumor Cells
Isolation Method:Depletion, positive isolation for molecular applications
Output Viability:>95%
Product Line:DYNAL®, Dynabeads®
Product Size:5 mL
Sample Type (Specific):Blood, PBMC
Starting Material (Cell #):1x10^7 PBMCs per isolation
Target Species:Human
Shipping Condition:Room Temperature


  • 产品描述
  • 产品功能
  • 产品参数

Description

Dynabeads® CD45 are superparamagnetic beads covalently coupled to anti-human CD45 antibody that enable isolation or depletion of CD45+ leucocytes directly from whole blood, buffy coat, or MNC suspensions. Dynabeads® CD45 can also be used to enrich epithelial tumor cells. The CD45 antigen is weakly expressed on myeloid cells. To efficiently deplete or isolate all leucocytes including myeloid cells, use Dynabeads® CD15 (Cat. No. 11137D) in combination with Dynabeads® CD45. Advantages of Dynabeads® CD45:

• Efficient depletion of human leucocytes
• Isolation of leucocytes directly from whole blood for molecular applications
• Enrichment of circulating epithelial tumor cells

About Dynabeads® CD45
Dynabeads® CD45 are uniform, superparamagnetic beads (4.5 µm diameter) coated with a primary monoclonal mouse IgG2a antibody specific for a CD45 membrane antigen common to all known isoforms of CD45. CD45 is expressed on all human leucocytes. Because of the bead size, Dynabeads® CD45 can easily and efficiently isolate or deplete cells from viscous samples such as whole blood and bone marrow in about 30 minutes. Dynabeads® CD45 is often used for enrichment of circulating non-hematopoietic tumor cells, by depletion of all CD45+ leucecytes from a MNC sample. Positively isolated cells can be used for downstream molecular studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that intact cells will not be released from these beads, and we do not recommend to analyze the bead-bound cells with a flow cytometer.

Magnetic bead–based separation offers easy handling
Dynabeads® CD45 is added to the sample under continuous mixing to optimize the binding of the Dynabeads® to the target cells. By placing the sample on a magnet it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, buffy coat, bone marrow, PBMC, or tissue digests.


Specifications

Capacity:Processes ~5x10^8 cells total
Cell Type:Leukocytes, Tumor Cells
Isolation Method:Depletion, positive isolation for molecular applications
Output Viability:>95%
Product Line:DYNAL®, Dynabeads®
Product Size:5 mL
Sample Type (Specific):Blood, PBMC
Starting Material (Cell #):1x10^7 PBMCs per isolation
Target Species:Human
Shipping Condition:Room Temperature


Specifications

Capacity:Processes ~5x10^8 cells total
Cell Type:Leukocytes, Tumor Cells
Isolation Method:Depletion, positive isolation for molecular applications
Output Viability:>95%
Product Line:DYNAL®, Dynabeads®
Product Size:5 mL
Sample Type (Specific):Blood, PBMC
Starting Material (Cell #):1x10^7 PBMCs per isolation
Target Species:Human
Shipping Condition:Room Temperature


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