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1
2019 - 03 - 08
1 月 28 日,国际学术期刊 Circulation Research 在线发表了中国科学院上海营养与健康研究所宗耕课题组与哈佛大学公共卫生学院营养系合作的最新研究成果 “Associations of Monounsaturated Fatty Acids from Plant and Animal Sources with Total and Cause-Specific Mortality in Two US Prospective Cohort Studies”。该研究发现较高的植物来源单不饱和脂肪酸(MUFA-Ps)摄入量与较低的总死亡率相关,而动物来源单不饱和脂肪酸(MUFA-As)的摄入则与较高死亡率相关。另外,用植物来源单不饱和脂肪酸替代饱和脂肪酸(SFAs)、精加工的碳水化合物或反式脂肪可以显著降低死亡风险。在全球范围内,心血管疾病(CVD)和癌症是造成过早死亡的最主要原因。目前,越来越多的研究表明采取健康的生活方式(如戒烟、增加体力活动和提高膳食质量等)能够有效降低过早死亡的风险。多个国家的膳食指南均强调了膳食脂肪的质量对预防慢性疾病的重要性,尤其提倡植物油及其他植物来源的脂肪摄入。例如,膳食中的多不饱和脂肪酸(PUFAs)主要来自植物,而现有的研究表明 PUFAs 能够降低心血管疾病风险和死亡率的关系。与之相比,饮食中的单不饱和脂肪酸只有一半来自植物油(尤其...
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2019 - 07 - 05
据新华社电 美国疾病控制和预防中心研究团队发现,正在刚果(金)使用的两种试验性疗法对抗击当前的埃博拉疫情有效。7 月 9 日发表在英国《柳叶刀—传染病》杂志上的研究显示,在实验室中,抗病毒药物 Remdesivir,以及由三种抗体组成的抗埃博拉药物 ZMapp 可阻断新一轮埃博拉疫情的致病毒株在人类细胞中增长。确认抗病毒药物有效的关键之一在于找到此次疫情的致病毒株。论文第一作者、美疾控中心微生物学家劳拉 · 麦克马伦说,目前在刚果(金)试用的疗法均针对以前疫情中获取的埃博拉毒株,而埃博拉是一种核糖核酸(RNA)病毒,会不断变异,因此确定现有疗法是否对新毒株奏效至关重要。美疾控中心研究人员没有当前在刚果(金)传播的 “伊图里” 毒株样本,但他们从开源基因库中获得病毒序列数据,使用 “反向遗传学” 技术重组了毒株。这项技术有助于研究人员更多了解新毒株在埃博拉家族树中的位置,从而寻找更多潜在疗法,并测试各类新疗法的有效性,还可与未来的新毒株进行比对。研究中还发现,2014 年到 2016 年西非多国爆发埃博拉疫情期间开发的一种实验室病毒诊断方法可用于诊断 “伊图里” 毒株。(周舟)文章摘自:生物360
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2020 - 02 - 21
2020年2月16日讯 /生物谷BIOON /--国家指南建议前列腺癌患者多吃蔬菜,这可能会减少癌症的进展和死亡。但在一项III期随机临床试验中,前列腺癌患者被指定每天吃7份或更多的蔬菜和水果,却没有发现额外的微量营养元素摄入的保护作用。'这些数据表明,尽管科学和公众舆论普遍认为需要吃蔬菜,但多吃蔬菜缺确实不会改变前列腺癌的病程。据我们所知,它不会抑制或治愈它。然而,虽然多吃水果和蔬菜的健康饮食和多做运动不一定能治愈癌症,但它可能使身体更强壮、更健康,这可能有助于患者忍受癌症治疗。'图片来源:Wikipedia这项研究近日发表在《Journal of the American Medical Association》上,,由加州大学圣地亚哥分校Moores癌症中心和Roswell Park综合癌症中心的研究人员完成,招收了478名年龄在50到80岁男性。这些患者被诊断为早期前列腺腺癌,并参与了一个积极的监测项目,在该项目中,患者推迟立即治疗,直到病情恶化。患者被随机分为两组,一组接受关于饮食和前列腺癌的书面信息,另一组接受电话咨询的行为干预项目,该项目鼓励参与者食用类胡萝卜素含量高的食物,如绿叶蔬菜、胡萝卜和西红柿,以及十字花科蔬菜,如西兰花和卷心菜。两组都接受了两年的监测。被分配到干预组的患者增加了水果和蔬菜的摄入量,达到了有统计学意义的程度,明显多于对照组患者。...
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时间: 2019 - 04 - 22
描述The LIVE/DEAD™ Fixable Green Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. This kit has been optimized and validated for use with a blue laser flow cytometer.• Stable—dyes are freeze dried in separate vials to maintain stability• Robust—staining pattern is the same before and after fixation• Bright signal—allows...
型号:
时间: 2019 - 04 - 18
描述This product detects the externalization of phosphatidylserine in apoptotic cells using recombinant annexin V conjugated to green-fluorescent FITC dye and dead cells using propidium iodide (PI). Propidium iodide stains necrotic cells with red fluorescence. After treatment with both probes, apoptotic cells show green fluorescence, dead cells show red and green fluorescence, and live cells show little or no fluorescence.
型号:
时间: 2019 - 04 - 18
描述SYTOX® Green nucleic acid stain is an excellent green-fluorescent nuclear and chromosome counterstain that is impermeant to live cells, making it a useful indicator of dead cells within a population.•  Impermeant to live cells•  Excitation/Emission: 504/523 nm•  Use with 488 Argon-ion laser•  Works with mammalian cells and both Gram-positive and Gram-negative bacteriaSimple Viability Determination SYTOX® Green allows quick determination of cell viability when usin...
型号:
时间: 2019 - 04 - 18
描述Premo™ FUCCI Cell Cycle Sensor is a fluorescent, two-color sensor of cell cycle progression and division in live cells. It allows accurate and sensitive cell cycle analysis of individual cells or a population of cells by fluorescence microscopy, flow cytometry, or high-content imaging.At 2 x 200 µL, this is a smaller pack size version (1/5 volume) of the popular full-size product and perfect for testing its performance in your system.The Premo™ FUCCI Cell Cycle Sensor is delivered by high...
型号:
时间: 2019 - 04 - 18
描述CountBright™ absolute counting beads are a calibrated suspension of microspheres that are brightly fluorescent across a wide range of excitation and emission wavelengths (UV to 635 nm excitation and 385-800 nm emission). CountBright™ absolute counting beads are mixed with the cell sample and assayed via flow cytometry. By comparing the ratio of bead events to cell events, absolute numbers of cells in the sample can be calculated. Because CountBright™ beads are mixed in the test sample, absolut...
型号:
时间: 2019 - 04 - 17
描述CellTrace™ CFSE Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry.• Superior performance—bright, single-peak staining enables visualization of multiple generations• Long-term signal stability—well-retained in cells for several days post stain• Non-toxic—staining does not adversely effect cell health• Simple, robust staining protocolView a selection guide for all CellTrace™ Cell Proliferation Kits fo...
型号:
时间: 2019 - 04 - 17
描述CellTrace™ CFSE 细胞增殖试剂盒 (CellTrace™ CFSE Cell Proliferation Kit) 以方便的一次性瓶装形式提供用途广泛,稳定性高的细胞示踪剂。 羧基荧光素二醋酸盐琥珀酰亚胺酯,常称为 CFSE,可被动扩散进入细胞。 这种试剂没有颜色和荧光,直至细胞内的酯酶去除其醋酸盐基团,产生能发出明亮荧光的羧基荧光素琥珀酰亚胺酯。 琥珀酰亚胺酯与细胞内氨基反应,形成稳定且能用醛固定剂固定的荧光偶联物。 此试剂盒包含十份一次性瓶装的 CFSE,因此进行小规模实验时无需制备过量的易变质 CFSE 储存液。 此试剂盒还包括高质量的 DMSO(二甲基亚砜)和详细的实验方案。For Research Use Only. Not for use in diagnostic procedures.图表Suppressive capacity of isolated Treg cells.Suppressive capacity of isolated Treg cells. (A) CD4+ CD25–cells were stained with Ca...
型号:
时间: 2019 - 04 - 17
描述The Click-iT® EdU Pacific Blue™ Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells as compared to traditional BrdU methods. Newly synthesized DNA is analyzed using the 405 nm laser of the flow cytometer.• Accurate—superior results compared to BrdU assays• Fast—results in as little as 90 minutes• Economical—more assays per kitView selection guide for all Click-iT™ EdU and Click-iT™ Plus EdU assays for flow c...
型号:
时间: 2019 - 04 - 17
描述The 2018 Attune NxT Acoustic Focusing Cytometer has expanded functionalities to drive your research forward. Designed and developed to remove common barriers associated with flow cytometry, the evolutionary capabilities of the Attune NxT flow cytometer offer adaptable optical configuration options so you can get the most out of your multicolor analysis.• Free Attune NxT software with researcher-inspired upgrades• New green laser option for expanded utility and fluorochrome options• Simple push...
型号:
时间: 2019 - 04 - 17
描述The 2018 Attune NxT Acoustic Focusing Cytometer has expanded functionalities to drive your research forward. Designed and developed to remove common barriers associated with flow cytometry, the evolutionary capabilities of the Attune NxT flow cytometer offer adaptable optical configuration options so you can get the most out of your multicolor analysis.• Free Attune NxT software with researcher-inspired upgrades• New green laser option for expanded utility and fluorochrome options• Simple push...
型号:
时间: 2019 - 04 - 17
描述The 2018 Attune NxT Acoustic Focusing Cytometer has expanded functionalities to drive your research forward. Designed and developed to remove common barriers associated with flow cytometry, the evolutionary capabilities of the Attune NxT flow cytometer offer adaptable optical configuration options so you can get the most out of your multicolor analysis.• Free Attune NxT software with researcher-inspired upgrades• New green laser option for expanded utility and fluorochrome options• Simple push...
型号:
时间: 2019 - 04 - 17
描述The 2018 Attune NxT Acoustic Focusing Cytometer has expanded functionalities to drive your research forward. Designed and developed to remove common barriers associated with flow cytometry, the evolutionary capabilities of the Attune NxT flow cytometer offer adaptable optical configuration options so you can get the most out of your multicolor analysis.• Free Attune NxT software with researcher-inspired upgrades• New green laser option for expanded utility and fluorochrome options• Simple push...
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